反复热性惊厥大鼠海马白细胞介素-6和肿瘤坏死因子-α水平的变化

Changes of Interleukin-6 and Tumor Necrotic Factor-α Levels in Hippocampus of Rats with Frequent Febrile Seizures

目的探讨反复热性惊厥(FS)大鼠海马IL-6和肿瘤坏死因子-α(TNF-α)的变化。方法将43只SD大鼠随机分为正常对照组(n=10,NC组)、高热对照组(n=12,HC组)和热性惊厥组(n=21,FS组)。分别用酶联免疫吸附法(ELISA)和逆转录酶多聚链反应(RT-PCR)检测大鼠海马IL-6和TNF-α水平及IL-6 mRNA、TNF-αmRNA水平。结果1.FS与HC组大鼠海马IL-6水平[分别为(53.21±8.32)ng/mg、(56.37±2.84)ng/mg]明显高于NC组[(44.55±4.11)ng/mg](P<0.01,0.05),FS与HC组比较差异无统计学意义(P>0.05)。各组大鼠海马IL-6 mRNA水平间比较差异无统计学意义(Pa>0.05)。2.各组大鼠海马TNF-αmRNA及其蛋白水平比较均无统计学意义(Pa>0.05)。结论反复FS对大鼠海马IL-6和TNF-α表达无明显影响,IL-6和TNF-α可能不参与FS相关脑损伤的产生过程。

Objective To explore the changes of interleukin - 6 （ IL - 6） and tumor necrotic factor -α（ TNF -α） levels in hippocampus of experimental model rats with frequent febrile seizures （FS）. Methods Forty - three rats were randomly divided into 3 groups： normal control group （ n = 10, NC group ） , hyperthermic control group （ n = 12, HC group） and febrile seizures group （ n =21, FS group）. The mRNA and protein levels of IL - 6 and TNF -α in hippocampus of these rats were determined by reverse - transcription polymerase chain reaction （RT-PCR）and enzyme -linked immunosorbent assay （ELISA） respectively. Results 1. IL -6 in hippocampus FS group[（53.21 ± 8.32 ） ng/mg] and HC group[ （ 56.37 ± 2.84 ） ng/mg ] were significantly higher than that of NC group[ （44.55 ± 4.11 ） ng/mg] （ P 〈 0.01,0.05 ）. There was no significant difference in hippocamps IL- 6 level between FS group and HC. group（P 〉 0.05 ）. There was no significant difference in IL - 6 mRNA in hippocamps between various groups （ P, 〉 0.05 ）. 2. There was no significant difference in TNF -α mRNA and protein level in hippocamps between various groups （ Pa 〉 0.05 ）. Conclusions Frequent FS might not change the expression levels of IL - 6 and TNF -α in hippocampus. IL - 6 and TNF -α might not play a role in development of FS induced brain damage.