云南省1997-2000年及2004年非脊髓灰质炎肠道病毒分子生物学及流行特征分析

Study on the molecular typing and epidemiology of non-polio enteroviruses isolated from Yunnan province,China

目的描述云南省1997-2000年及2004年5年的肠道病毒（EV）分离状况及型别特征。方法5年在急性迟缓性麻痹病例中共检测到210株非脊髓灰质炎（脊灰）肠道病毒（NPEVs）,其中12株经血清学鉴定为腺病毒。剩余198株经基因测序定型,即病毒VP1区基因序列转变为氨基酸序列后与标准株进行比较。结果经分子生物学定型：人类EV-A组5株（5个血清型）、EV-B组158株（34个血清型）、EV-C组32株（6个血清型）、未分离到EV-D组病毒。在急性迟缓性麻痹病例监测中,210个分离株,EV-B组病毒占75．2%,为主要型别;EV-C组占12．2%,腺病毒占5．7%,EV-A组占2．4%。结论云南省EV的流行中,以EV-B组较多。

Objective This report presented an overview on the epidemiology of enterovirus in Yunnan province, the People＇s Republic of China. Methods A total of 210 strains of non-polioviruses isolated under acute flaccid paralysis surveillance during a 5-year study period from 1997 to 2000 and 2004 were examined. Of the 210 non-polioviruses strains, a total of 12 strains of adenoviruses were serologically identified. The remaining 198 isolates were used for molecular typing, and the viral genomes of 195 non-polio enteroviruses（NPEVs） were translated to corresponding amino acid sequences and compared with those of the prototype strains. Results Based on molecular typing, 5 isolates were classified into 5 serotypes of human enterovirus A species while 158 isolates into 34 serotypes of B and 32 isolates into 6 serotypes of C species. However, we did not isolate any viruses which belonged to human enterovirus D species. Thus, under acute flaccid paralysis surveillance, human enterovirus B species accounted for 75.2 % of the 210 isolates and was considered as the predominant one, followed by human enterovirus C（12.2 % ）, adenovirus （ 5.7 % ）, and human enterovirus A （ 2.4 % ）. Conclusion Although the epidemiological characteristics of NPEVs from Yunnan province remained ＂unknown＂, the molecular typing method had provided us a breakthrough to understand the epidemiology of these viruses.