RP-HPLC法测定布洛芬离子导入凝胶剂的含量

Determination of Ibuprofen in Ionphoretic Gel by RP-HPLC

目的 建立一种测定离子导入凝胶剂中布洛芬含量的高效液相色谱法。方法 采用反相高效液相色谱（RP—HPLC）法，以Shim-pack VP—ODS柱（4．6mm×150mm，5μm）作分析柱，流动相为甲醇-水-磷酸（75：25：0．8，v／v／v），流速1．0ml／min。柱箱温度40％，检测波长为264nm。结果 本法的日内RSD为0．35％，日间RSD为0．68％，平均回收率为100．4％。标准曲线在10-80μg·ml^-1浓度范围内，呈现良好的线性关系（r=0．9993）。结论 该法用于布洛芬离子导入凝胶剂的含量测定，快速、准确、可行。

Aim To establish an accurate method for the determination of ibuprofen in ionphoretic gel . Methods RP-HPLC was adopted with Shim-pack VP-ODS （4.6mm × 150mm ,5 μm）as the analytical column, methanol-waterphosphate acid（75： 25： 0.8, v/v/v）as the mobile phase. The flow rate was 1.0ml/min. Column temperature was at 40 ℃. Detection wavelength was at 264nm. Results Inter-day RSD of ibuprofen was 0.35% and intra-day less than 0.68% ,respectively. The average recovery rate of ibuprofen was 100.4%. The calibration curve was liner ranging from 10 -80μg·ml^-1（ r = 0.999 3 ）. Conclusion This method was found to be sensitive, rapid and accurate, and suitable for the determination of ibuprofen in the preparation.