串珠素在大鼠牙胚发育过程中的表达和意义

The perlecan expression in the dental follicle development

目的:观察牙胚发育不同时期串珠素(Perlecan)的分布与表达情况,探讨其与牙胚发育的关系。方法:出生0天、1天、1周、2周的SD大鼠取下颌第一磨牙,行Perlecan免疫组化染色(SABC法),光镜观察其表达与分布情况。结果:Perlecan在大鼠各时间段牙胚均有阳性表达,但各时期的分布模式不同,出生后各时间段,成釉器中均为强阳性表达,而牙囊中Perlecan表达随着牙胚发育而不断增强,之后又随着牙胚的成熟而下降。结论:Perlecan可能在牙胚的发育过程中发挥着重要的调节作用。

Objective To observe the distribution and expression of Perlecan in the dental follicle in the different developmental stages. Methods The rat＇s dental follicle of the 1st mandibular molar was taken from the 0day, one day, one weeks and 2weeks newborn rats. The samples were stained with the immunocytochemistry of SABC. The distribution and expression of perlecan was analyzed with image analysis system. Results Perlecan was expressed in the developmental stages of dental follicle.But the distribution patterns at various stages were differens. The results of image analysis showed the positive expression at one day and one week samples was stronger than that at Oday and 2 week samples. Conclusion The expression of perlecan is related to the development of dental germ and may play a role in the extralcellular matrix formation of the dental follicle primary developmental statues. Its expression reduced with the mature of dental germ matrix.