无患子愈伤组织诱导的多因子正交试验研究

Studies on Callus Induction in Sopindus mukorossi Gaertn by Orthogonal Design

目的：探寻无患子愈伤组织诱导的最佳外植体、培养基和培养条件.方法：采用5因素混合水平正交设计,用DPS2000统计软件对其结果进行方差分析和多重比较（SSR）.结果：诱导愈伤组织的最佳外植体为当年生枝条水培芽的嫩茎段,用0.2%HgCl2消毒2min,其无菌存活率为98.33%,培养条件为光照或黑暗,最佳培养基配方为：MS（B5）＋6-BA 1.5mg·l^-1＋NAA0.2mg·l^-1,其愈伤组织的诱导率为97.78%.结论：激素配比是影响愈伤组织诱导的主要因素.较高的6-BA浓度（1.5～2.5 mg·l^-1）与较低的NAA浓度（0.2mg·l^-1）配比,有利于无患子愈伤组织的诱导.

Objective：It was to search after the optimal explants,medium and condition of callus inducement of Sopindus mukorossi Gaertn.Methods： The Orthogonal design L8（4×2^4） was used in this experiment and the statistic software DPS2000 was employed to analyse the result＇s variance and multiple comparisons.Results： The results showed that the tender stem on the twig which grew in sterile water was best explants,which was sterilized with 0.2% HgCl2 for 2min.Its surviving rate was 98.33%;Inducing condition was darkness or light; the optimal medium was MS （B5） ＋ 6- BA 1.5mg·l^-1 ＋ NAA 0.2mg·l^-1 , On which callus induction rate was 97.78%. Conclusiou： The influence of the combination of 6 - BA and NAA on callus induction was a key. A considerable 6- BA concentrafion（1.5 ～2.5mg·l^-1 ）in combination with a little NAA（0.2mg·l^ -1 ） would avail callus induction.