摘要
本文使用免疫细胞化学PAP法和计算机图家分析技术,对动脉粥样硬化大鼠下丘脑弓状核ACTH免疫反应阳性细胞的变化进行了半定量研究,并对其阳性细胞的形态、数目和分布在光镜下进行了观察。结果表明:实验组,弓状核ACTH免疫反应阳性神经元比对照组明显减少(P<0.05),阳性细胞的积分光密度,实验组和对照组分别为0.1028±0.031和0.1250±0.051,本文从形态学角度提示了弓状核ACTH免疫反应阳性细胞对动脉粥样硬化的形成可能有一定影响。
The changes of adrenocorticotropic hormone immunoreactive (ACTH-in) neurons of the hypothalamic arcuate nucleus in rats with atherosclerosis was studied semiquantitativly by immunocytochemical peroxidase-antiperoxidase (PAP) method and image analysis. The morphology, distribution n and number of ACTH-ir neurous were observed under microscope. The results showed that the number of ACTH-ir neruons in the experimental group was significantly lower than that in the control group. The average of the integral optical density (IOD) of positive weurons was 0.1028± 0.031 add 0.1250± 0.051 respectively in the experimental and control groups. From morphological point of view, it is suggested that the ACTH-ir peurons in the arcuate nucleus might have influence on development and/ or control of atherosclerosis.
出处
《神经科学》
SCIE
CAS
1996年第2期64-67,共4页
Chinese Journal of Neuroscience