低氧对间充质干细胞的影响

Effect of Hypoxia on Mesenchymal Stem Cells——Review

氧对于几乎所有的生命都是必需的,它维持能量代谢及细胞的功能,但机体多种细胞是处于生理或病理性低氧状态中,而体外细胞实验一般是在20%氧浓度下进行,并不符合生理状态。直到最近,关于低氧对于间充质干细胞(mesenchymal stem cells,MSC)的影响有了一系列的研究。体外实验表明,适度低氧下MSC的生长和存活能力更好,氧浓度可以影响MSC向成骨、成软骨、成脂的分化倾向,低氧能提高特异性受体和配体相结合所介导的迁移。低氧可影响胚胎干细胞、造血干细胞、神经干细胞的生理学特性也是一证据。干细胞对缺氧反应的分子机制主要涉及低氧诱导因子-1(hypoxic inducible factor-1,HIF-1)信号通路。本文综述了低氧对MSC的存活、增殖、分化和迁移的影响,以及涉及HIF-1等的分子机制。

Oxygen is issential for life,but cultivation of cells is usually performed under 20% 02, that do not replicate normal physiological hypoxia or pathological hypoxia conditions in the body. Recently, the effect of hypoxia on mesenchymal stem cells （MSCs） has been studied, under physiological hypoxia, MSCs thrive well, and the ability differentianing to osteoblast, chondrocyte and adipocyte as well as the ability of migration are changed. Hypoxia changes the physiological characteristics of embryonic stem cell, hematopoietic stem cell and neuron stem cell as well, The mechanism of these responses might be primarily involved in the hypoxic inducible factor-1 （HIF-1） signal pathway, This review emphasizes that hypoxia is an important factor on all major aspects of stem cell biology including survival, proliferation, differentiation, and migration, and the mechanism involved in HIF-1 signaling pathway behind these responses was also discussed.