期刊文献+

黄芪对SGC7901胃癌细胞COX-1、COX-2、VEGF和PGE_2表达的影响 被引量:39

Effect of Astragalus membranaceus on expression of COX-1,COX-2,VEGF,and PGE-2 in human gastric cancer cell line SGC7901
下载PDF
导出
摘要 目的:观察黄芪对人胃癌SGC7901细胞中COX-1、COX-2、VEGF和PGE_2表达的影响。方法:采用MTT试验观察黄芪对胃癌细胞的抑制作用,用RT-PCR以及Western blot方法研究加药后人胃癌细胞COX-1、COX-2基因及其蛋白表达的变化。用ELISA方法检测培养基中VEGF、PGE_2的表达情况。结果:黄芪能抑制人胃癌细胞生长,呈一定的量效特征;并能抑制人胃癌细胞COX-2、VEGF和PGE_2的表达。结论:黄芪抗肿瘤的机制可能是通过抑制COX-2,进而抑制其下游产物PEG_2的表达及使VEGF表达下调,从而抑制肿瘤的生长。 Objective;To observe the effect of Astragalus membranaceus on expression of cyclooxygenase-1 (COX-1), COX- 2, vascular endothelial growth factor (VEGF), and prostaglandin E2 (PGE-2) in human gastric cancer cell line SGC7901. Methods: The inhibitory effects of Astragalus membranaceus on viability of SGC7901 cells was asessed by MTT assay. The expression of COX-1 and COX-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. The expression of VEGF and PGE-2 in culture medium was detected by ELISA. Results:Astragalus membranaceus inhibited growth of SGC7901 cells in a dose-dependent manner and down-regulated expressions of COX-2, VEGF, and PGE-2. Conclusion:The anti-tumor mechanism of Astragalus membranaceus is related with inhibition of COX-2 expression, which causes the suppression of its downstream product PGE-2 expression and down-regulation of VEGF, therefore decreases tumor growth.
出处 《肿瘤》 CAS CSCD 北大核心 2007年第3期194-198,共5页 Tumor
基金 江苏省自然学科基金资助(编号:BK2001186)
关键词 胃肿瘤 黄芪 药物作用 血管内皮生长因子类 SGC7901细胞 Stomach neoplasms Astragalus membranaceusl ,drug effeet Vascular endothelial growth factors SGC7901 cells
  • 引文网络
  • 相关文献

参考文献10

  • 1WILLAMS C S,DDBOIS R N.Prostaglandin indoperoxide synthase:why two is oforms?[J] Am J Physiol,1996,270(3):393-400.
  • 2FOSSLIEN E.Molecular pathology of Cyclooxygenase-2 in neoplasia[J].Ann Clin Lab Sci,2000,30(1):3-21.
  • 3SUNG J J,LEUNG WK,GO M Y,et al.Cyclooxygenase-2 expression in helicobacter pylori-associated premalignant and malignant gastric lesions[J].Am J Pathol,2000,157 (3):729-735.
  • 4ZARIDZE D,BORISOVA E,MAXIMOVITCH D,et al.Aspirin protects agaist gastric cancer:results of a case-control study from Moscow,Russia[J].Int J Cancer,1999,82(4):473-476.
  • 5AWAOKA H,KAWANO S,TSUJI S,et al.Effects of NSAIDs on proliferation of gastric cancer cells invitro:possible implication of cyclooxygenase-2 in cancer development[J].J Clin gastroenterol,1998,27(Suppl 1):47-52.
  • 6IKEGUCHI M,ODA S,SAIO H,et al.The expression of vascular endothelial growth factor and proliferative activity of cancer cells in gastric cancer[J].Langenbecks Arch Surg,1999,384(3):264-270.
  • 7SENGER DR,van de WATER L,BROWN LF,et al.Vascular permeability factor (VPF,VEGF) in tumor biology[J].Cancer Metastasis Rev,1993,12 (3/4):303-324.
  • 8李凤朝,孔庆兖,杨雪梅.血管内皮生长因子在胃癌及不典型增生组织中的表达及意义[J].徐州医学院学报,2004,24(3):221-223. 被引量:1
  • 9吴开春 邓敬兰 张学庸.胃癌组织PGE产生及其对PHA介导的3H-TdR掺入的抑制.第四军医大学学报,1988,9(5):295-296.
  • 10CORREA P.Human gastric carcinogenesis:a multistep and multifactorial process-First American Cancer Socioty award lecture on Cancer Epide miology and Prevention[J].Cancer Res,1992,52(24):6735-1280.

二级参考文献5

  • 1Maeda K, Kang SM, Onoda N, et al. Expression of p53 and vascular endothelial growth factor associated with tumor angiogenesis and prognosis in gastric cancer[J]. Oncology, 1998, 55(6) :594 - 599.
  • 2Senger DR, Van de Water L, Brown LF, etal. Vascular permeability factor (VPF, VEGF) in tumor biology [J]. Cancer Metastasis Rev,1993,12(3 - 4) :303 - 324.
  • 3Ikeguchi M, Oka S, Saito H, et al. The expression of vascular endothelial growth factor and proliferative activity of cancer cells in gastric cancer[J]. Langenbecks Arch Surg, 1999, 384(3):264-270.
  • 4Kido S, Kitadai Y, Hattori N, et al. Intedeukin 8 and vascular endothelial growth factor in human gastric carcinomas? [J]. Eur J Cavcer, 2001,37(12): 1482 - 1487.
  • 5KakejiY, KogaT, SumiyoshiY, etal. Clinicalsignificanceofvasvular endothelial growth factor expression in gastric cancer [J]. J Exp Clin Cancer Res , 2002 , 21 (1):125- 129.

同被引文献536

引证文献39

二级引证文献441

;
使用帮助 返回顶部