摘要
目的:观察黄芪对人胃癌SGC7901细胞中COX-1、COX-2、VEGF和PGE_2表达的影响。方法:采用MTT试验观察黄芪对胃癌细胞的抑制作用,用RT-PCR以及Western blot方法研究加药后人胃癌细胞COX-1、COX-2基因及其蛋白表达的变化。用ELISA方法检测培养基中VEGF、PGE_2的表达情况。结果:黄芪能抑制人胃癌细胞生长,呈一定的量效特征;并能抑制人胃癌细胞COX-2、VEGF和PGE_2的表达。结论:黄芪抗肿瘤的机制可能是通过抑制COX-2,进而抑制其下游产物PEG_2的表达及使VEGF表达下调,从而抑制肿瘤的生长。
Objective;To observe the effect of Astragalus membranaceus on expression of cyclooxygenase-1 (COX-1), COX- 2, vascular endothelial growth factor (VEGF), and prostaglandin E2 (PGE-2) in human gastric cancer cell line SGC7901. Methods: The inhibitory effects of Astragalus membranaceus on viability of SGC7901 cells was asessed by MTT assay. The expression of COX-1 and COX-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. The expression of VEGF and PGE-2 in culture medium was detected by ELISA. Results:Astragalus membranaceus inhibited growth of SGC7901 cells in a dose-dependent manner and down-regulated expressions of COX-2, VEGF, and PGE-2. Conclusion:The anti-tumor mechanism of Astragalus membranaceus is related with inhibition of COX-2 expression, which causes the suppression of its downstream product PGE-2 expression and down-regulation of VEGF, therefore decreases tumor growth.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第3期194-198,共5页
Tumor
基金
江苏省自然学科基金资助(编号:BK2001186)
关键词
胃肿瘤
黄芪
药物作用
血管内皮生长因子类
SGC7901细胞
Stomach neoplasms
Astragalus membranaceusl ,drug effeet
Vascular endothelial growth factors
SGC7901 cells