糖胺多糖含量在腭裂发生机制中的意义

Significance of glycosaminoglycans content in developing mechanism of cleft palate

为探讨糖胺多糖在胚胎腭器官发育和腭裂发生机制中的意义，本实验对妊娠１２０天小时的ＮＩＨ系小白鼠腹腔内注射５０ｍｇ／ｋｇＤｅｘａｍｅｔｈａｓｏｎａｃｅｔａｔｅ诱导腭裂胚胎模型，通过标准位的颅颌冠状切片，经阿辛蓝／ＰＡＳ组织化学染色，用计算机ＴＤＳ－９０像片数字化系统对切片图像腭突中糖胺多糖（ＧＡＧｓ）进行面积的相对定量分析。结果显示，腭突内阿辛蓝着色在胚龄１３１２天小时标本中开始出现，此时，正常组ＧＡＧｓ面积量为４．６９ｍｍ２，给药组为２．４０ｍｍ２，两组差别统计学有显著性意义（Ｐ＜０．０５）。随着胚胎的发育，腭突内ＧＡＧｓ面积量逐渐增加，但给药组始终表现比正常组少（Ｐ＞０．０５）。证明腭裂的发生与早期腭突内ＧＡＧｓ含量的降低有关。

To explore significance of Glycosaminoglycans content in the development of embryo and forming mechanism of cleft palate,Dexamethason acetate was injected into the abdomen of NIH mouse on the 12th day of gestation to induce cleft palate mouse embryo model Then by taking cranio maxillary coronary sections ,stained with Alcian blue/PAS and quantitatively analysing the area of the palatal process covered by glycosaminoglycans using a TDS 90 photodigital system with computer Results indicate that on 13 12 day hour of gestation the colour of Alcian blue starts to show in the palatal process By then,the area covered by glycosaminoglycans in the normal group is 4 69mm whereas for the experimental group is 2 40mm, showing a significant statistical difference( P <0 05) The area of the palatal process covered by glycosaminoglycans gradually increases as the embryo developes,but is distinctly smaller in experimental than in the normal group ( P >0 05) This proves that there′s a relationship between the level of glycosaminoglycans in the palatal process and development of cleft palates