摘要
利用免培养法(Culture independent approach)提取浓香型白酒曲药中细菌的总DNA,在分子水平上运用PCR扩增技术和16S rDNA序列同源性分析等方法测定曲药中细菌的16SrDNA基因近全序列,并建立系统发育树图。结果表明,组成该曲药中的细菌分属于Delftia、Dys-gonomonas、Bacteroidetes、proteobacterium、Nocardiopsis、Pseudomonas和Arthrobacter几大类群(phylum),表现出高度的细菌多样性。
In order to know the bacterial composition in Luzhou-flavor liquor Quyao, the techniques including culture independent approach, PCR technique and 16S rDNA sequence homology analysis etc. were used to measure 16S rDNA gene total order of bacteria in Quyao and the phylogenetic tree was further established. According to the sequences alignment and through the analysis of its comparability, the bacteria of Luzhou-flavor Quyao were consisted of Delfiia, Dysgonomonas, Bacteroidetes, proteobacterium, Nocardiopsis, Pseudomona and A rthrobacter, which indicated their bacterial diversity.
出处
《酿酒科技》
北大核心
2007年第5期17-19,共3页
Liquor-Making Science & Technology
基金
四川省经贸委重点项目(No.川经贸技术2002-236)
关键词
微生物
大曲
16S
RDNA序列
PCR扩增
免培养法
细菌
microbe
Luzhou-flavor liquor quyao
16S rDNA sequence
PCR
Culture independent approach
bacteria