舟山地区嗜盐菌的分离和产胞外多糖菌株的筛选

Isolation and exopolysaccharide screening of halophiles from Zhoushan Islands

对浙江舟山地区近海和盐田的样品进行分离检测.从11份泥样和16份水样中共分离纯化120株嗜盐菌,其中多数为中度嗜盐菌或耐盐菌,颜色以白色为主,其次是红色和黄色,细胞形态多为杆状或球状,革兰氏阴性菌株占81.6%.研究表明,嗜盐菌在该地区具有广泛的分布和一定的多样性.以分离菌株为材料筛选产胞外多糖的菌株,发现19株能产生胞外多糖,其中菌株HS239产量较高.

Totally 120 halophilic strains were isolated from mud and water samples from Zhoushan Islands. Most of the isolates are moderately halophilic or halotorant, in which, white colonies are dominant species while red and yellow colonies are also popular. Cells of the isolates are mainly cocci or rod-shaped and approximately 81.6% of the strains are Gram-negative. The results indicated that the communities of halophiles are widely distributed in Zhoushan Islands and possess diversity. All the isolates were screened for EPS, among which 19 strains showed positive in EPS-producing. The strain HS239 displayed high EPS productivity.