分离检测生物活性物质的荧光标记试剂与分子探针及其应用

Fluorescent Labeling Reagents and Molecular Probes Used in Separation and Detection of Bioactive Substances

作者结合自己的研究工作主要评述了用于分离检测氨基化合物(氨基酸、肽、蛋白质和生物胺等)、巯基化合物(谷胱甘肽、半胱氨酸和高半胱氨酸等)及NO等生物活性物质的荧光标记试剂和荧光分子探针的近期进展和应用。除了传统的OPA、NDA、DNS、FMOC、FITC、NBD-F、AQC、Cy5等在HPLC和CE分离荧光检测应用新进展,还介绍了许多新的荧光分子探针和标记试剂的性能和应用,它们是N-羟基琥珀酰亚胺活性酯荧光标记试剂,6-氧-(N-琥珀酰亚胺乙酸酯)-9-(2′-甲氧羰基)荧光素(SAMF),1,3,5,7-四甲基-8-苯基-(4′-O-(N-琥珀酰亚胺乙酸酯))-二氟化硼-二吡咯甲烷(TMPAB-OSu,)等和3-碘乙酰胺苯嵌蒽酮荧光探针及MCY5、DSTCY、DCDSTCY和DCTCY等花菁类近红外荧光探针等。

Integrated with the authors＇ work, the recent development and application of the fluorescent labeling reagents and molecular probes for the determination of amino acids, peptides, proteins, biological amines, thiol compounds and NO etc, are reviewed. Besides OPA, NDA, DNS, FMOC, FITC, NBD-F, AQC, Cy5, some recent fluorescent label reagents and molecular probes are also discussed with respect to their applications in the separation and fluorescent detection of HPLC or CE. They are N-hydroxysuccinimidyl ester fluorescent labeling reagent, 6-oxo-（ N- hydroxysuccinimidyl ＂acetic acid ester ）-9-（ 2＇-methyloxocabonyl ） fluorescein, 8-phenyl-（ 4＇-oxy-acetic acid N- hydroxysuccinimidyl ester）-4, 4-difluoro-1, 3, 5, 7-tetramethyl-4-bora-3a, 4a-diaza-S-indacene （TMPAB-OSu） etc , fluorescent probe 3-iodoacemidobenzanthranone and near-infrared fluorescent probe MCY5, DSTCY, DCDSTCY and DCTCY.