钙处理对乙烯诱导的番茄离体花柄脱落的抑制作用

Physiological Regulation of Calcium in Tomato Pedicel in Vitro During Abscission Induced by Ethylene

以番茄‘辽园多丽’的小花为材料,研究外源乙烯(20μL.L-1)处理下,花柄脱落过程中,离区微粒体膜Ca2+-ATPase、羧甲基纤维素钠酶(CMC-Na)、内切多聚半乳糖醛酸酶(Endo-PG)和果胶脂酶(PE)等活性的变化。结果表明:外源乙烯加速了花柄的脱落,刺激了其离区的Ca2+-ATPase活性先升高后下降,随后离区CMC-Na、Endo-PG、PE活性升高;钙离子专一性螯合剂EGTA和钙调素拮抗剂TFP均在一定程度上抑制了乙烯诱导的上述反应,表明细胞内Ca2+与CaM参与了乙烯反应。

In an experiment, pedicels of Lycopersicon esculenturm Mill. ‘ Liaoyuanduoli＇ were used to study the changes of Ca^2＋ -ATPase activity, abscission rate and the activity of Endo-PG, PE, CMC-Na enzymes in abscission zone after the pedicels were incubated in exogenous ethylene （20 μL · L^-1 ）. The results showed that ethylene could accelerate the abscission during incubation, Ca^2＋ -ATPase activity first rose sharply and then declined quickly. Subsequently, there was a increase in the activity of Endo-PG, PE, CMC-Na enzyme in abscission zone. Calmodulin antagonist trifluoperizine （TFP） and Ca^2＋ chelator EGTA both inhibited the effects induced by ethylene to a certain extent. It is concluded that Ca^2＋ and CaM participate in the reaction of exogenous ethylene.