摘要
为阐明小鼠胚胎期皮肤的神经末梢是否与羊水有接触关系及胚胎期皮肤一氧化氮合酶(NOS)阳性神经末梢的分布和发育状况,利用逆行轴浆运输的原理将荧光金在体注射到胚胎的羊水中,用荧光显微镜观察神经元被标记情况。同时分别取胚胎11天、13天、15天、17天、19天、21天和出生后1天的小鼠的四肢,冰冻切片,做NADPH-d酶组织化学法,在光镜下观察并摄片。实验结果显示,在脊神经节等部位发现有荧光金标记的神经细胞,主要为圆形或不规则形。而皮肤NOS阳性神经末梢在小鼠胚胎11天出现表达,并随时间而增加,主要位于表皮层,直接裸露在羊水中。结果提示胚胎期羊水与皮肤神经末梢有一定的接触关系,有可能参与胎儿神经末梢的生长发育,羊水中物质是否对周围神经有生长诱向作用值得进一步探讨。
The purpose of the present study was to explore whether the skin has the connection with the amniotic fluid and the distribution and development of nitric oxide synthase (NOS) positive nerve terminals in the skin of mouse embryo. Fluorogold (FG) was injected into amniotic fluid in mice with 13-15 d pregnancy in vivo, observing whether they have FG in somatic nerves and dorsal root ganglion cells of fetal by light microscope, and the acral skin tissue of embryo day 11 (E11), embryo day 13 (E13), embryo day 15 (E15), embryo day 17 (E17), embryo day 19 (E19), embryo day 21 (E21) mice embryos and postnatal day 1(P1) mouse were taken out, then the frozen sections of acral skin tissue of each group were stained with NADPH-diaphorase (NADPH-d) enzymohistochemistry method to localize the expression of NOS. Our results showed that in the spinal nerves or other nerves of fetal, some FG positive round or irregular neural cells were observed. FG mainly in the cell body, with even and bright in the cytoplasm, and the boundary was clear. While the expression of NADPH-d positive nerve terminals began to appear in Ell embryos, mainly in the cuticular layer, and exposure to the amniotic fluid directly. The expression intensity were steadily increasing along with the development of the embryo, and became-similar on E21 to that on P1. It is inferred that in the embryonic period, the amniotic fluid may be in touch with the skin of fetal, so the amniotic fluid may take part in the growth and development of nerve terminals of fetal. It need further discussion whether the substance in amniotic fluid have the effect of inducing growth on the development of peripheral nerve.
出处
《细胞生物学杂志》
CSCD
2007年第2期277-281,共5页
Chinese Journal of Cell Biology