黑色素生物合成抑制剂MA-18化学结构和生物学活性的研究

MA-18,a melanin biosynthesis inhibitor-production,isolation, physico-chemical properties,structure and biological activity

从棘孢小单孢福建亚种No．80－A18（Micromonosporaechinosporasubsp，fujianen－sisNo80－A18）的发酵液中提取的黑色录生物合成抑制剂MA－18，经提取、纯化和理化性质鉴别，根据元素分析、EI－MS和13C-NMR的结果，确定其分子式为C7H7NO2，分子量137，分析1H-NMR和13C－NMR主要信号的归属．推测MA－18为苯的衍生物。MA－18抑制轮生链霉菌（Str－eptoverticilliummelosporm）80－A16在胨铁球脂培养基（ISP6）、青色链霉菌（Streptomycesglau－cus）93－5在酪氨酸琼脂培养基（ISP7）上形成黑色素，并抑制蘑菇酪氨酸酶合成黑色素蛋白。MA－18不抗真菌和大多数细菌，仅对金葡球菌有微弱作用．

MA-18, a melanin biosynthesis inhibitor, was isolated from the fermenta-tion broth of Micromonospora echinospora subsp. fujianensis No. 80-A18. Its molecularformular, C7H7NO2(MW 137), was determined by elemental analysis, EI-MS and 13C-NMRspectrum, MA-18 was considered as a benzene derivative on the basis of its data of H-NMRand 13C-NMR.The melanin biosynthesis inhibitor, MA-18, inhibited the production of hydrogensulifide by Streptoverticillium melosporum 8o-A16 and inhibited the activity of tyrosinase byStreptomyces glaucus 93-5 and by mushroom respectively, and also showed weaklyantibacterial activity against Staphylococcus aureus.