铝螯合剂对染铝大鼠肾功能及必需元素的影响

Effect of aluminum chelating agent on renal function and essential elements of aluminum-induced rats

目的:探讨铝螯合剂1,2-二甲基-3-羟基-4-吡啶酮(1,2-dimethyl-3-hydroxy-4-pyridone,DHPO)对铝染毒大鼠血清肌酐、尿素氮、尿酸及肾脏中铝、锌、铜、铁、钙、镁等元素的影响。方法:30只雄性Wistar大鼠随机分为6组,阴性对照组、铝染毒组、预防组及低、中、高剂量组,AlCl3染毒3周后分别给予不同剂量的DHPO 1周,测定血清肌酐、尿素氮、尿酸含量及肾脏中铝、锌、铜、铁、钙、镁等元素的含量。结果:铝染毒组尿素氮含量显著高于阴性对照组(P<0.05),其余各组与阴性对照组相比差异均无统计学意义;各组大鼠血清中肌酐、尿酸含量比较差异均无统计学意义。铝染毒组肾脏中Al含量显著高于阴性对照组(P<0.01),低、中、高剂量组均显著低于铝染毒组(P<0.01),且中、高剂量组与阴性对照组相比差异均无统计学意义;铝染毒组、低剂量组大鼠肾脏中铜含量均显著低于阴性对照组(P<0.01,P<0.05),而中、高剂量组与阴性对照组相比差异无统计学意义;低、中、高剂量组的大鼠肾脏中锌、铁、钙、镁的含量与阴性对照组相比差异均无统计学意义。结论:DHPO在促排铝的同时,对肾脏中锌、铜、铁、钙、镁含量无影响,且能使受损的肾功能得到恢复。

Objective： To investigate the effects of 1,2-dimethyl-3-hydroxy--4-pyridone （DHPO） on creatinine（Car）, urea nitrogen（BUN） and uric acid（UA） in serum and on aluminum（M）, zinc（Zn）, copper（Cu）, iron（Fe）, calnum. Methods： Wistar ratscium（Ca） and magnesium（Mg） in the kidneys of rats induced by alumi were subjected to aluminum chloride for 3 weeks. Then they were treated with different doses of DHPO. The content of Cr, BUN and UA in the serum was determined, and the concentrations of Al, Zn, Cu, Fe, Ca and Mg in kidney tissues were determined. Results： The BUN content of the AI group was significantly lower than that of the negative control group. The result of UA and Cr contents demonstrated that there was no statistical significance among the groups. The Al level in the kidneys was significantly increased with an intake increase. The aluminum concentration of the three DHPO groups was significantly lower than that of the Al group（ P 〈 0.01 ）. The aluminum concentration of the moderate and high DHPO groups was not significantly different from that of the negative control group. The Cu concentration of the Al group and the low DHPO group was significantly lower than that of the negative control group, whereas there was no statistical significance between the moderate and high DHPO groups and the negative control group. Compared with the negative control group, the Zn, Fe, Ca, Mg concentrations of the low, moderate and high DHPO groups were not significantly different. Conclusion： DHPO could effectively promote the elimination of Al in kidneys, protect the kidney from Al-induced damage, and has no effect on the excretion of Zn, Cu, Fe, Ca and Mg.