摘要
目的:建立测定穿山龙中薯蓣皂苷元含量的反相高效液相色谱法,从而为控制穿山龙的质量提供参考。方法:采用双相酸水解法提取穿山龙中的薯蓣皂苷元;使用 Agilent Zorbax 色谱柱(4.6 mm×250 mm,5 μm),以乙腈-水(94:6)为流动相,检测波长为203 nm,流动相流速为1.0 mL·min^(-1),柱温为40℃。结果:样品中的薯蓣皂苷元可以与各干扰组分达到基线分离;用本法测定薯蓣皂苷元的线性范围为1.055~21.10 μg;重复性试验(n=6)RSD=1.7%;平均回收率(n=6)达到96.8%,RSD=1.5%;试验中测得穿山龙中薯蓣皂苷元的平均含量(n=6)为1.61%,RSD=1.7%。结论:本方法具有供试品溶液制备简便,检测灵敏,线性范围宽,重复性好等优点,适合于穿山龙中薯蓣皂苷元的含量测定。
Objective:To establish an RP - HPLC method for determination of diosgenin in Rhizoma Dioscoreae Nipponicae for quality control. Methods : Diosgenin was extracted by two - phase acid hydrolysis from the dried material ; Agilent Zorbax Extend C18 column (4.6 mm × 250 mm, 5μm) was adopted at 40℃ with acetonitrile - pure water(94: 6)as mobile phase. The detection wavelength was 203 nm,and the flow rate was 1.0 mL· min^-1. Results:Diosgenin could be separated obviously from the intefferential components in the sample. The linearity of the calibration curve (r = 0. 9999)was good in the range of 1. 055 -21.10μg for diosgenin, RSD of repeated experiments ( n = 6) was 1.7 %, with the average recovery ( n = 6 ) reached 96.8 % ( RSD = 1.5 % ). The average content ( n = 6) of diosgenin in Rhizoma Dioscoreae Nipponicae was 1.61% ( RSD = 1.7 % ). Conclusions: This method can sensitively detect the diosgenin with a simple pretreatment, and has a broad linear range with good repeatability, which makes it being fit for determination of diosgenin in Rhizoma Dioscoreae Nipponicae.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第5期635-638,共4页
Chinese Journal of Pharmaceutical Analysis