摘要
在单细胞植株再生基础上建立起体细胞无性系变异体系。本文是在前文研究基础上,以25个单细胞无性系为试验材料,进一步对无性系后代穗部产量构成性状进行分析。结果表明,穗部产量性状变异范围较广,有利的变异类型在第4代稳定遗传,并已繁殖到第8代。1994年和1995年优良稳定株系进行田间品种比较试验,初步筛选出有希望直接应用于生产的株系。
The somaclonal variation system was established on the base of plant regeneration from single cell culture in vitro in wheat. Yield component characters of main spike from regenerated plant derived from single cell culture were studied further. Twenty-five somaclones were used in the studies. The results showed that the variation of yield component characters of main spike was significant and the range of variation was wider. The valuable variation could be heritable and stable in the fourth generation and these somaclones with the valuable variation have been propagated till the eighth generation. The comparative experiment of field cultivars of the stable somaclones were carried out in 1994 and 1995. The preliminary results showed that some of them could be applied in production directly. This indicated that the induction and screening of somaclones derived from single cell culture is an effective way improving yielding characteristics in wheat.
出处
《东北农业大学学报》
CAS
CSCD
1997年第1期1-8,共8页
Journal of Northeast Agricultural University
关键词
小麦
单细胞培养
体细胞无性系
变异
性状变异
wheat, single cell culture, somaclonal variation, yielding component characteristics