腹腔液中CD44和整合素β1表达与胃癌腹膜转移的关系

Expression of CD44 and integrin β1 in peritoneal lavage fluid and their correlations with the peritoneal metastasis of gastric cancer

目的:探讨腹腔液中CD44和整合素β1 mRNA表达与胃癌腹膜转移的关系.方法:收集50例胃癌患者的腹腔液标本,用RT-PCR法检测其中CD44和整合素β1 mRNA的表达,同时行腹腔液细胞学(PLC)检查.按病灶浸润深度、浆膜类型、PLC检查结果、腹膜转移情况和TNM分期分组,比较不同组之间CD44和整合素β1 mRNA的表达水平.结果:腹腔液CD44 mRNA表达水平与浆膜类型(F=4.59,P<0.05)、腹膜转移有关(t=2.655,P<0.05),与PLC检查(t=3.126,P<0.01)和TNM分期密切相关(t=3.453,P<0.01,);腹膜转移组的整合素β1 mRNA表达明显高于未转移组的水平(t=2.241,P<0.05).结论:腹腔液中CD44和整合素β1 mRNA表达与胃癌腹膜转移相关因素及TNM分期密切相关,检测其水平有助于估计胃癌病程进展和患者预后,对腹膜转移的早期诊断也有一定价值.

AIM： To investigate the expression of CD44 and integrin 91 mRNA in peritoneal lavage fluid and their correlations with the peritoneal metastasis of gastric cancer. METHODS： The samples of peritoneal lavage fluid were collected during operation from 50 patients with gastric cancer. The expression of CD44 and integrin β1 was detected at mRNA level by reverse transcription-polymerase chain reaction （RT-PCR）, and the examination of peritoneal lavage cytology （PLC） was also performed. All the cases were divided into different groups according to the invasion depth of foci, serosal types, results of PLC, peritoneal metastasis and TNM stages. Then the expression of CD44 and integrin β1 mRNA was compared among various groups. RESULTS： The expression of CD44 mRNA was closely related to serosal types （F = 4.59, P 〈 0.05）, peritoneal metastasis （t = 2.655, P 〈 0.05）, results of PLC （t = 3.126, P 〈 0.01） and TNM stages （t = 3.453, P 〈 0.01）. Integrin β1 mRNA expression was significantly higher in patients with peritoneal metastasis than that without metastasis （t = 2.241, P 〈 0.05）. CONCLUSION： The expression of CD44 and integrin β1 mRNA in peritoneal lavage fluid is related to the peritoneal metastasis-related factors and TNM stages of gastric cancer, detection of their levels is helpful to evaluate the progression, prognosis and early diagnosis of gastric cancer.