甜樱桃砧木‘Colt’小茎尖离体再生体系的建立

Establishment of the Minishoot-Tip Culture System in Vitro in Sweet Cherry Rootstock Colt'

[目的]建立甜樱桃砧木Colt的离体培养体系.[方法]采取小茎尖离体培养的方法.[结果]在茎尖分化培养中,合适的培养基为1/2MS+1.0 mg/L BA+0.1 mg/L IBA.在增殖培养中,经过筛选认为,最佳培养基为1/2MS+1.0 mg/L BA+0.2 mg/L IBA+0.5 mg/L GA3,增殖系数高,植株生长势旺,增殖阶段最适的pH值为5.2～6.0.最适的蔗糖浓度是2%～3%.生根培养阶段,培养基1/2MS+0.5 mg/L IBA上试管苗的生根效果最好,生根率高.[结论]对茎尖培养各个阶段的多种影响因素组合进行了研究,初步建立了一套较为完整的甜樱桃砧木Colt的小茎尖培养体系.

[ Objective ] To establish the technique systems of minishoot-tip culture in Colt. [ Methods ] The regeneration euhure of minishoot-tip was used. [ Results ] Factors influencing propagation were optimized and discussed, A relatively optimal culture system for Colt shoot-tips is established. The optimized medium for shoot-tips initial culture was 1/2MS ＋ 1.0mg/L BA ＋0. 1 mg/L IBA. In subculture muhiplifieation,the optimized medium was 1/2MS ＋ 1, 0 mg/L BA ＋0. 2 mg/L IBA ＋0. 5 mg/L GA3in which the propagation ratio is h/gh and the v/gor of trees is strong. The su/table value of pH is 5, 2 - 6. 0,Suitable density of sugar is 2% - 3%. The optimized medium of rooting was 1/2MS ＋0.5 mg/L IBA in which the ratio of roofing was high and roots grew quickly and strongly. [ Conclusion] Based on the study of many factors,the systems of minishoot-tip culture in Colt was established.