HPLC测定七花活血片中人参皂苷Rg_1、Rb_1的含量

Determination of the Contents of Ginsenoside Rg_1,Rb_1 in Qihua Huoxue Tablet by HPLC

目的:建立七花活血片的含量测定方法。方法:采用HPLC梯度洗脱法测定七花活血片中人参皂苷Rg_1、Rb_1的含量。色谱柱:lichrospherC_(18)柱(4．6mm×150mm,5μm);流动相:乙腈-水(20:80～40:60)线性梯度洗脱;流速:0．8mL·min^(-1);柱温:30℃,检测波长:203nm。结果:人参皂苷Rg_1的进样量在1．025～10．25μg线性关系良好,r=0．999 4,平均回收率为96．61%,RSD=1．26%;人参皂苷Rb_1的进样量在0．545～5．45μg线性关系良好, r=0．999 3,平均回收率为96．51%,RSD=1．94%。结论:本法准确、专属性强、重现性好,可作为本制剂的含量测定方法。

Objective： To establish a determination method for Qihua Huoxue tablet. Methods： The content of Ginsenoside Rg1, Rb1 in Qihua Huoxue Tablet was detected by HPLC gradient elution method. HPLC was performed with Lichrospher C18 column （4.6mm×150mm, 5μm） with a mobile phase of acetonitrile -water of （20： 80 -40：60） gradient elution and the detection wavelength was at 203nm, the flow rate being 0. 8 mL·min^-1; the injection volume was 10μL; the column temperature was 30℃. Results： Ginsenoside RgI has a good linearity within 1. 025 - 10. 25μg （r =0. 999 4） and the average recovery was 96.61% with RSD = 1.26% ; Ginsenoside RbI has a good linearity within 0. 545 -5.45μg （r = 0. 999 3） and the average recovery was 96.51% with RSD = 1.94%. Conclusion： This method is accurate, sensitive, reproucibile and exclusive, is available for quality of Qihua Huoxue tablet.