辛伐他汀在人骨髓基质干细胞向成骨细胞分化过程中的作用

The effect of simvastatin on the osteoblastic differentiation of human bone marrow stromal cells

目的观察辛伐他汀对体外培养的人骨髓基质干细胞(Human Bone Marrow Stromal cells,hBMCs)成骨分化功能的影响,探讨其刺激成骨的作用机制。方法体外培养来自于外伤所致股骨颈骨折患者的骨髓基质干细胞,传代后实验组加入1×10-7mol/L的辛伐他汀,在不同时间点采用ELISA检测核转录因子1(Core Binding Factor1,Cbfa1)与DNA的结合活性,碱性磷酸酶(Alkaline Phosphatase,ALP)比活性,及放射免疫法检测骨钙素(Osteocalcin,OCN)含量。结果在辛伐他汀作用后,实验组与对照组比较,实验组Cbfa1因子与DNA的结合活性增高,ALP比活性增高且骨钙素含量增加。结论1×10-7mol/L辛伐他汀能够促进人骨髓基质干细胞成骨分化,此种促进作用可能与辛伐他汀增加其分化过程中相关转录因子的活性有关。

Objective To investigate the effect of Simvastatin on function of osteoblastic cell differentiation of human bone marrow stromal cells （hBMCs）in vitro, and to study the mechanisms of anabolic effect of Simvastatin on bone formation. Methods hBMCs from patients underwent artificial femoral head replacement after femoral neck fracture was cultured in vitro. After being added with 1×10^-1 mol/L of Simvastatin, the changes of Cbfa 1 activity were examined by ELISA, the changes of alkaline phosphatase （ALP） activity were examined by ALP t Kit, and the changes of osteocalcin were examined by radioimmunologic assay. Results After the supplementation of Simvastatin, the activity of Cbfa 1 in hBMCs increased, and the level of ALP and osteocalcin （OCN） also increased compared with the control group which did not receive Simvastatin. Conclusion Simvastatin can stimulate osteoblastic differentiation with increased ALP activity and OCN production while promoting the activity of Cbfa 1 in vitro. These results may be parts of the mechanisms of anabolic effect of Simvastatin on bone formation.