枸杞对酒精性肝损伤大鼠保护作用的实验研究

The Study of Protection Function of Lycium Barbarrum on Alcoholic Liver Injury in Rats

目的观察宁夏枸杞对酒精性肝损伤大鼠的保护作用。方法选择成年雄性SD大鼠60只,体重300-340g,随机分为四组：空白对照组每天给予蒸馏水灌胃,喂饲基础饲料;其余三组按10mL/（kg.bw）剂量给予56度白酒灌胃,酒精模型组喂饲基础饲料;低剂量枸杞组：喂饲基础饲料＋枸杞（50g/kg）;高剂量枸杞组：喂饲基础饲料＋枸杞（100g/kg）,喂饲3周,实验期末,空腹12h,20%乌拉坦麻醉后,心脏取血制备血清及10%肝组织匀浆,测定谷丙转氨酶（ALT）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、总抗氧化能力（T-AOC）,并取肝组织病理切片检查。结果与酒精模型组比较,枸杞组肝脏SOD、CAT活性均高于酒精模型组,差别有统计学意义（P〈0.01）;枸杞组血清ALT活性低于酒精模型组,差别有统计学意义（P〈0.01）;枸杞组血清、肝脏中MDA含量均低于酒精模型组,差别有统计学意义（P〈0.05）;肝组织病理结果提示宁夏枸杞对酒精性肝损伤大鼠具有保护作用。结论宁夏枸杞对酒精性肝损伤大鼠具有抗脂质过氧化及保护肝细胞的作用。

Objective To explore the protection effects of Lycium batbarrum（LB） on alcoholic liver injury in rats. Methods Sixty male rats weighed 300-340g were randomly divided into four groups. The blank group were given distilled water and basic animal feeds. The other three groups were given 56 degrees spirit by 10ml/ kg· bw. The alcohol model rats were fed with basic animal feeds. The low dose group rats were fed with basic animal feeds and LB 50g/kg. The high dose group rats were fed with basic animal feeds and LB 100g/kg. After three weeks, we took serum from heart and liver organization to detect the ALT, SOD, CAT, MDA, T-AOC and check the pathological slice of liver organization. Results Compared with the alcohol model group, LB group＇s activity of SOD, CAT were significantly higher. The activity of serum ALT in LB group were significantly lower than that in the alcohol model group （ P 〈 0.01 ）. The concentration of MDA in the alcohol model group were significantly lower than that in the alcohol model group （ P 〈 0.05）. The pathological results showed that LB had certain protection effect on alcohol liver injury in rats. Conclusion LB has lipid per-oxidation and protection effects for rats with alcohol liver injury.