人类巨细胞病毒感染与结肠癌相关关系的初步研究

Preliminary Study of Relationship Between Human Cytomegalovirus Infection and Colon Carcinoma

目的探讨HCMV感染与结肠癌的相关关系,为结肠癌的防治提供新思路。方法利用ELISA技术检测结肠癌患者和正常对照组HCMV-IgG及IgM,同时利用PCR技术及免疫组织化学技术检测结肠癌组织及结肠癌癌旁组织HCMVDNA及HCMVpp65抗原的表达情况,并对这些结果进行分析。结果HCMV-IgG阳性率在结肠癌组和正常对照组无显著性差异。结肠癌组HCMV-IgM检出率明显高于正常对照组;HCMVDNA在结肠癌组织及结肠癌癌旁组织的检出率无显著性差异,但HCMVpp65抗原表达在结肠癌组织明显增强,且无年龄组差异,HCMVpp65表达增强主要见于结肠腺癌。结论HCMV感染与结肠癌的发生明显相关,且HCMV导致结肠癌发生有赖于HCMV的复制。

Objective To explore the relationship between human cytomegalovirus （HCMV） infection and colon carcinoma. Methods The detection of HCMV- IgG and IgM in serum of colon carcinoma patients and healthy control group by ELISA, HCMV genome DNA by PCR and the expression of HCMV pp65 by immunohistochemical technique in cancerous and noncancerous tissues were carried out and analyzed. Results HCMV- IgG positive rate in the two groups showed no significant difference each other （X^2 =2. 0917, P〉0.05）, while the positive rate of HCMV- IgM in carcinoma group was significantly higher than that in the controls （X^2 = 4. 5737, P〈 0.05）. There was also no statistical difference in HCMV genome DNA positive rate of cancerous and noncancerous tissues （X^2 = 0. 3356, P〉0.05）, while the expression of HCMV pp65 was significantly higher in cancerous tissues than that in noncancerous tissues （X^2 = 7. 6008, P〈0.05）, and the enhanced expression of HCMV pp65 was mainly found in colon adenocarcinoma. Conclusion HCMV infection closely relates to colon carcinoma, and the colon cancer caused by HCMV is dependent on the reproduction of the virus.