组织芯片研究Notch1与NF-κB在胃癌中的表达和意义

Expressions and clinical significance of Notchl and NF-κB in gastric cancer

目的:研究Notchl和NF-KB在胃癌中的表达与临床病理特征的关系以及两者间相互作用的关系。方法:通过免疫组化的方法检测了含有168例胃癌和27例正常胃组织的组织芯片中Notchl和NF-κB的表达情况。结果:Notchl在胃癌中表达较正常胃组织明显上调(P<0.01),并与肿瘤大小、分化程度、浸润深度、脉管浸润明显相关;NF-κB在胃癌中表达明显高于正常对照组,并与肿瘤大小、分化程度、浸润深度、远处转移及脉管浸润明显相关。Kaplan-Meier分析显示Notchl和NF-κB阳性组的预后较阴性组差。Cox回归多因素分析显示Notchl是影响胃癌预后的独立因素之一(P<0.05)。结论:NF-κB的过度表达可能参与了Notchl信号通路失调介导的胃癌发生发展机制。Notchl可能成为评价胃癌预后和生存的新的指标,也可能作为胃癌治疗的新靶点。

Objective：To investigate the expression of Notchl and NF-κB in gastric cancer and discuss the association between the expressions of both and their relationship with the clinicopathological features. Methods： The expression of Notchl and NF-κB in 168 specimens of gastric cancer and 27 specimens of normal gastric tissues were detected by SP immunohistochemistry. Results：The expression of Notchl was significantly upregnlated in gastric cancer as compared with normal gastric tissues （ P 〈0. 01 ）. It was closely related with tumor size, differentiation grade, depth of invasion, and vessel invasion. The expression of NF-κB in gastric cancer was remarkably higher than that in control group （ P 〈0.01 ） and was significantly associated with tumor size, differentiation degree, depth of invasion, distant metastasis, and vessel invasion. Kaplan-Meier analysis showed that Notchl- and NF-κB positive patients had poor prognosis than Notchl- and NF-κB-negative patients. Cox regression multivariate analysis showed that Notchl was one of the independent prognostic factors for gastric cancer （P 〈0.05 ）. Conclusion：The over-expression of NF-κB may be involved in the carcinogenesis and progression of gastric cancer mediated by Notchl signaling pathway. Notchl may become a potentially useful marker for evaluating the prognosis and survival of gastric cancer and a new therapeutic target.