期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNAi的机制及在医学研究中的应用 被引量：2

The Mechanism of RNAi and its Application in Medical Research
下载PDF
导出
摘要 RNAi干扰即一些小双链RNA可高效、特异地阻断体内特定基因的表达,导致特异mRNA降解,从而引起生物体内特异基因的沉默,使细胞表现出某种基因表型的缺失。近年来,RNAi这种技术作为一种简单有效的代替基因敲除的遗传工具在医学研究中得到了广泛的应用并有望成为攻克病毒、肿瘤等疾病的有效手段。 Small double-stranded RNA-mediated interference （ RNA1 ） can target complementary homologous mRNAs for degradation via a mechanism termed RNAi. It can efficiently induce gene-specific silencing. As a powerful reverse genetic tool to replace gene knockout, RNAi technology has been used broadly in medical research and may direct treatment of viral diseases and cancer.
作者 星懿展 李泽桂
机构地区 第三军医大学细胞生物教研室 第三军医大学组织胚胎学教研室
出处 《解剖科学进展》 CAS 2007年第2期169-172,共4页 Progress of Anatomical Sciences
基金 国家自然科学基金资助项目(30170983)
关键词 RNAI 医学研究 特异基因 小双链RNA 生物体内 RNA降解 基因表型 基因敲除
分类号 R394 [医药卫生—医学遗传学]
  • 相关文献

参考文献22

  • 1Fire A,Xu S,Montgomery MK,et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J].Nature.1998.391:806-811.
  • 2Hannon GJ.RNA interference[J].Nature,2002,418(6894):244-251.
  • 3Silva JM,Hammond SM,Harmon GJ.RNA interference:a P romising app roach to antiviral therapy[J]?Trends in Molecular Medicine,2002,8(11):501-508.
  • 4Harborth J,Elbashir SM,Bechert K,et al.Identification of essential genes in cultured mammalian cells using small interfering RNAs[J].Journal of Cell Science,2001,114(24):4557-4565.
  • 5Bernstein E,Caudy AA,Hammond SM,et al.Role for a bidentate ribonuclease in the initiation step of RNA interference[J].Nature,2001,409,363-364.
  • 6Elbashir,S.M.et al.(2001)Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate[J].EMBO J.20,6877-6888.
  • 7Elbashir SM,Lendeekel W,Tuschl T.RNA inter-ference is mediated by 21～and 22～nucleotide RNAs[J].Genes Dev,2001,15(2):188-200.
  • 8Guangchao Sui,Christina Soohoo,El Bachir Affar,et al.A DNA vector-based RNAi technology to suppress gene expression in mammalian cells[J].PNAS,2002,99(8)5515-5520.
  • 9Sui G,Soohoo C,Affar EIB,et al.A DNA vector-based RNAi technology to suppress gene expression in mammalian cells[J].Proc Natl Acad Sci USA,2002,99(8):5515-5520.
  • 10Hannon GJ.RNA interference[J].Nature.2002,418(6894):244-251.

同被引文献18

  • 1仲明,张宏福,董枫岚,孙绍光,王润平,杨琳.RNAi技术及其在畜牧业中的应用前景[J].中国畜牧兽医,2006,33(8):46-49. 被引量：1
  • 2Fire A,Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J]. Nature, 1998 (391) :806 - 811.
  • 3Harmon G J. RNAinterference. Nature, 2002, 418 (6894):244-251.
  • 4Silva JM, Hammond SM, Harmon GJ. RNA interference:aPtom-ising approach to antir Mtherapy[J]. Trends in Molecular Medicine, 2002,8 (111) : 501 - 508.
  • 5Hammond S M, Bernstein E, Beach D, Harmon G J. An RNA directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature, 2000 (404): 293-296.
  • 6Hammond S M, Argonaute. a Link Between Genetic and Biochemical Analyses of RNAi. Science, 2001 (293):1146-1150.
  • 7Wassenegger M, Heroe S. RiedelL RNA-directed denovo methylation of genom is sequences in plants. Cell, 1994(76) :567-576.
  • 8Zamore P D, Tuschl T, Sharp P A. Bartel DP. RNA: double-stranded RNA directs theAfP- dependent cleavage of mRNA at 21 to 23 naeteofide intervals. Cell,2000. 101(1) :25-33.
  • 9ElbashirJ, Harborths M, LEedeckel W. Duplexes of 2 1 -nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature, 2001 (4 ! !) : 494- 498.
  • 10Clemena J C, Worby C A, Simonson-Lea N. Use of double stranded RNA interference in Drosophila cell lines to dissect signal transudation path Ways, Proc. Natl Acad Sci. USA,2000,97(2):6499-6503.

引证文献2

二级引证文献1

解剖科学进展
2007年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部