新抗生素M-90产生菌原生质体制备、再生及对产抗生素的影响

Protoplast Formation and Regeneration from the Micromonospora M 90 and the Effects on Antibiotics Yield

报道了新抗生素Ｍ－９０产生菌原生质体的制备、再生及对产抗生素的影响。利用二级菌丝培养，在３５℃、溶菌酶浓度为２ｍｇ／ｍｌ的条件下，酶解菌丝，形成原生质体达１０１１／ｍｌ；选择适当的再生条件，再生率达２０％；原生质体再生株的抗生素产量比对照株提高３４％。

The paper reports the formation and regeneration of protoplast from the new antibiotics, Micromonospora M 90, as well as the effects on antibiotics yield producing strain. Under the conditions of 35℃ and 2 mg/ml of lysozyme, the secondary mycelia of M 90 was cultured and enzymelysed, and was protoplast formed at 10 11 /ml; the regeneration rate reached to 20% under the selected regenera ting conditions; the antibiotics yield of the regenerated protoplast colony was increased by 34% as compared with the control.