百合花器官的组织培养

Tissue culture of lily floral organs

以帝伯、西伯利亚、铁炮、金百合4个品种的花器官为外植体,进行不同品种花器官及同一品种花器官不同部位的组培难易程度试验。结果表明,品种间诱导率存在差异,从易到难的排序为帝伯、西伯利亚、铁炮、金百合;同一品种不同部位的诱导率也存在差异,从易到难的排序为花丝、花托、子房、花冠、花柱、花药。在初代诱导培养基中,以M S+6-BA 0.5m g/L+NAA 0.5m g/L和M S+6-BA 1.0m g/L+NAA 0.5m g/L较好;在继代增殖培养基中,帝伯和西伯利亚的最适培养基均为M S+6-BA 1.0m g/L+NAA 0.2m g/L,而铁炮百合为M S+6-BA 1.0m g/L+NAA 0.5m g/L。

The floral organs of four lily varieties, Diber, Siberia, Lilium longiflorum and L. trompeten were selected as explants for in vitro culture to study the difference in inducing abilities of calli among different varieties and different parts of same floral organ in a variety. The results showed that the inducing rates were different in four varieties. Diber was the easiest variety for inducing, followed by Siberia, L. Longiflorum and L. trompeten. The induced rates of different parts of floral organ in the same variety were also different. Their sequence, from easy to hard, was filament, floral receptacle, ovary, corolla, style and anther. During the primary culture stage, the medium MS＋0. 5mg/L 6-BA＋0.5mg/L NAA and MS＋1.0mg/L 6-BA＋0.5mg/L NAA showed a better inducing ability in all varieties. In the subculture stage, MS medium supplemented with 1.0mg/L 6-BA and 0. 2mg/ L NAA was suitable for Diber and Siberia but MS medium supplemented with 1.0mg/L 6-BA and 0. 5mg/L NAA fitted for L. trompeten .