摘要
将辣根过氧化物酶(HRP)固定在二氧化钛(TiO2)纳米颗粒或纳米管修饰玻碳电极(GC)上,形成纳米TiO2微粒/HRP修饰GC电极和TiO2纳米管/HRP修饰GC电极.比较了HRP在纳米TiO2微粒、TiO2纳米管电极上的直接电子转移反应.实验表明,HRP在TiO2纳米管电极表面更能有效地促进它的电活性中心发生电子交换反应.此外,还测定了HRP标记抗体的电化学性能,为抗原抗体免疫反应信号的选择提供了参考依据.
Horseradish peroxidase immobilized on the surface of titanium dioxide nanoparticles and nanotube modified glassy carbon electrode. The HRP direct electron transfer reaction was compared between TiO2 nanoparticles and nanotube GC electrode. The results demonstrated that the HRP on TiO2 nanotube/GC electrode could catalyze electron exchange reaction between electroactivity center of HRP and electrode. And the research of antibody labeled HRP provides a selected signal for antibody-antigen immunoassay.
出处
《电化学》
CAS
CSCD
北大核心
2007年第2期140-144,共5页
Journal of Electrochemistry
基金
北京市自然科学基金(NO.6052002)资助
关键词
二氧化钛纳米管
电极修饰
直接电催化
辣根过氧化酶
titanium dioxide nanotube
chemically modified electrode
direct electrochemistry
horseradish peroxidase,
