缺血预处理对犬肺移植供肺组织腺苷酸含量的影响

NUCLEOTIDE LEVELS OF GRAFT LUNG FROM CANINE DONOR

用反向高效液相色谱法测定犬肺移植供肺离体冷缺血２ｈ后肺组织腺苷酸含量。结果显示缺血预处理组（阻断肺门１０ｍｉｎ后开放１５ｍｉｎ）供肺ＡＴＰ与ＡＭＰ含量和腺苷酸池水平显著高于对照组（Ｐ＜０．０５）。提示缺血预处理结合低温ＥＣＳ保存能降低犬离体供肺缺血期的能量消耗。

Twelve canine left lung allotransplantation were performed. In the ischemic preconditioning group (Group IP, n =6), left donor lung was preconditioned with 10 min ischemia followed by 15 min reperfusion using the occuluding left hilum before resection and cold perfusion. The control group (Group C, n =6) underwent the same treatment but without ischemic preconditioning. Adenine nucleotides (ATP,ADP,AMP) of the donor lung tissue were measured using rHPLC after 2hr of resection and cold perfusion with Euro Collins solution (ECS). The results showed that contents of ATP and total adenine nucleotides (TAN) were much higher in Group IP than in Group C (322.9±61.2, 942.9±134.5 and 200.0±50.0, 668.4±59.6μmol·g -1 respectively, P <0.05). Histologic examination of the donor lung in Group IP showed less damage than in Group C after 2hr of transplantation. The results suggest that IP combined with cold ECS perfusion can reduce the energy metabolism in canine donor lung.