氟西汀对海马神经元生长的影响

Effect of fluoxetine on the growth of the hippocampal neurons

目的研究不同浓度氟西汀对体外培养的新生大鼠海马神经元生长的影响。方法进行新生SD大鼠海马神经元原代培养,采用烯醇化酶(NSE)免疫组织化学和尼氏染色鉴定海马神经元。在培养3 d的海马神经元中加入不同浓度(1、10、20、40μmol.L-1)的氟西汀,并设正常对照组,48 h后观察细胞有突起的神经元数目、突起长度和胞体长径。研究氟西汀对海马神经元生长的影响。结果10μmol.L-1氟西汀组海马神经元与正常对照组比较,有突起的神经元数目增加、最长突起长度增长(P<0.05);40μmol.L-1氟西汀组海马神经元与正常对照组比较,有突起神经元数目减少(P<0.05),最长突起长度及胞体长径无统计学差异(P>0.05);1μmol.L-1、20μmol.L-1氟西汀组海马神经元与正常对照组比较,有突起神经元数目、最长突起长度及胞体长径无统计学差异(P>0.05)。结论一定浓度的氟西汀能促进海马神经细胞的生长。

Objective To explore the effects fluoxetine of different concentration on the growth of the hippocampal neurons of newborn rat. Methods We conducted primary culture of hippocampal neurons of newborn SD rat and identified the hippocampal neurons by NSE immuohistochemical staining and Nissl staining. The dif- ferent levels of fluoxetine（ 1,10,20 and 40 ·mol· L^-1 ）were added in the hippocampal neurons of cultured for 3 days and a control group was established. We measured the number of neurons with neurite and the length of neurites and the maximum diameter of neurons soma 48 hour later. Thus we could explore the effects of fluoxetine on the growth of the hippocampal neurons. Results The number of neurons with neurite and the length of neurites in 10 μmol· L^ -1 fluoxetine group were increased as compared with the control group（P 〈 0, 05 ） ;The number of neurons with neurite in 40 μmol· L ^-1 fluoxetine group were decreased as compared with the control group （ P 〈 0. 05） ;All of the indexes in 1,20 μmol·L^-1 fluoxetine group did not show significant difference as compared with the control group. Conclusion Appropriate concentration fluoxetine can promote the growth of hippocampal neurons.