重组生长激素对严重感染后蛋白质代谢影响的实验研究

Influence of recombinant growth hormone on protein metabolism during severe infection:an animal experiment

本文旨在探讨重组生长激素对腹腔严重感染后蛋白质代谢的影响及其作用机制。作者以大鼠盲肠结扎穿孔复制严重感染模型，并将其分为治疗组和感染组，分别于术后给予生长激素（ｒＧＨ）和生理盐水，进行观察研究。结果显示：（１）ｒＧＨ促进正氮平衡恢复，提高了血浆白蛋白水平；（２）ｒＧＨ促进肠粘膜谷氨酰胺利用酶活性的恢复，维持了肠粘膜正常形态结构，降低了门脉内毒素含量和循环ＴＮＦ水平；（３）ｒＧＨ直接促进了离体肝细胞合成白蛋白，感染时提高受抑的白蛋白ｍＲＮＡ表达水平。结果表明，ｒＧＨ可促进感染后肠粘膜对谷氨酰胺的利用，维持粘膜正常结构和功能，减轻了肠源性高代谢反应。同时，从分子水平揭示ｒＧＨ直接刺激肝细胞白蛋白ｍＲＮＡ的表达，促进了感染时白蛋白的合成，有利于正氮平衡的恢复，提高了生存率。

We observed the influence of recombinant growth hormone (rGH) on protein metabolism during sepsis and found its mechanisms.Cecal ligation and puncture were choosen to duplicate the severe infection model.Animals of therapy group received rGH 1U/kg/d after CLP operation,while sepsis group received normal saline.rGH accelerated regaining of the positive nitrogen equilibrium,improved plasma albumin level.rGH accelerated the recovery of intestinal mucosa glutaminase activity,preserved the normal structure of intestinal mucosa,reduced the portal venous endotoxin level and venous TNF level.rGH improved the albumin synthesis of isolated hepatocytes, and inhibited the expression of albumin mRNA level during severe infection.We conelude that rGH preserves the normal structure and function of intestinal mucosa during sepsis,and reduces gut origin hypermetabolism reactions.Moreover,rGH improves the synthesis of protein.