葡萄糖氧化酶在炭黑上的固定及直接电化学

Direct Electrochemistry and Immobilization of Glucose Oxidase on Carbon Black

经红外光谱和电化学测量证明,用简单的吸附法能将葡萄糖氧化酶(GOx)固定在炭黑(CB)表面.电化学测量表明,固定在CB上的GOx能进行准可逆的直接电化学反应,其式量电位(E0′)为-0.436V,在40-150mV·s-1范围内,不随扫描速率而变化.电化学反应速率常数(ks)为0.800s-1,比文献报道的大30多倍.而且,固定在CB上的GOx能保持其对葡萄糖氧化的生物电催化活性.即使在保存两周后,其电催化活性仅下降了5%,表明固定在CB上的GOx有良好的稳定性.

The IR spectroscopic and electrochemical measurements demonstrated that glucose oxidase （GOx） could be immobilized on the surface of carbon black （CB） using a simple adsorption method. The electrochemical measurements indicated that GOx immobilized on CB could undergo the quasi-reversible and direct electrochemical reaction and keep the bioelectrocatalytic activity for the glucose oxidation. Even after conservation for two weeks, its electrocatalytic activity decreased only 5%, illustrating the good stability of GOx immobilized on CB.