摘要
为探索木质素的生物降解新途径,采用平板显色法从三株白腐真菌中筛选出一株灵芝属漆酶高产菌,以农作物秸秆等为培养基进行固态发酵,该菌主要产漆酶和少量木质素过氧化物酶.在优化条件下,该菌发酵18d,漆酶活力最高达2056U/g干曲.该菌以玉米秸秆、油菜秸秆及稻草作为唯一营养源固态发酵30d,对玉米秸秆的木质素降解率最大,达到23.7%,油菜秸秆也达到了16.2%.
In order to probe into a new way for lignin biodegradation, one Ganoderma lucidum is selected to produce laccase from three fungal strains by the diagnostic methods of extracelluar oxidase mat. When it is supported with agricultural residues under solid-state fermentation, G. lucidum mostly produces laccase and a little lignin peroxidase. The maximum laccase yield of 2,056U/g is obtained from the culture grown with the optimum condition after 18 days. Secondly, three sorts of crop straw are used separately as only support substrate for G. lucidum to grow. After 30 days, the content of lignin in corn stalk declines 23.7%, and the figure for rape cane is 16.2%.
出处
《合肥学院学报（自然科学版）》
2007年第3期6-9,共4页
Journal of Hefei University :Natural Sciences
基金
安徽省自然科学基金项目(070413266X)
合肥学院博士基金项目(RC0016)资助
关键词
灵芝
固态发酵
漆酶
木质素生物降解
Ganoderma lucidum
solid-state fermentation
laccase
lignin biodegradation
