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富血小板血浆在组织工程骨血管化中作用的实验研究 被引量:6

Effect of platelet rich plasma on vascularization of tissue-engineered bone
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摘要 目的探讨富血小板血浆(platelet rich plasma,PRP)在构建血管化组织工程骨中的作用。方法培养犬骨髓基质干细胞(bone marrow stromal cell,BMSC),制备 PRP 及脱钙骨基质(demineralized bone matrix,DBM)。12只犬分为3组,每组4只。每只犬的背部分为4区,A、B 区植入 DBM-BMSC-PRP;C、D 区植入 DBM-BMSC。A、C 区植入物用背阔肌带血管肌筋膜包裹;B、D 区植入物用背部不带血管的浅筋膜包裹。于4、8、12周取出植入物检查。结果术后12周,各组4各分区新生骨面积百分比分别为:A 区(79.48±5.38)%,B 区(55.78±5.78)%,C 区(54.06±5.04)%,D 区(35.15±4.13)%;血管化程度分别为:A 区(4.39±0.65)%,B 区(3.03±0.49)%,C 区(3.37±0.56)%,D 区(1.40±0.22)%。结论 PRP 可促进组织工程骨的形成和血管化。 Objective To study the effects of platelet rich plasma (PRP) on vascularization of tissue-engineered bone. Methods Bone marrow stromal cell (BMSC) were isolated from iliac bone of dogs, PRP was obtained from the same dog and demineralized bone matrix (DBM) was prepared from homologous bone. Twelve dogs were divided into three groups and the back of each dog was divided into four areas. The DBM- BMSC-PRP was implanted in the area A and B ;the DBM-BMSC without PRP was implanted in the area C and D . The implants in the areas A and C were wrapped using myo-fascia with blood vessel of latissimus dorsi. The implants in the area B and D were wrapped using superfacial fascia of the back without blood vessel, The implants were taken out 4, 8 and 12 weeks later for examination. Results The degree of calcification and blood vessel formation of the implants was A 〉 B 〉 C 〉 D. Conclusions Both PRP and vessels of latissimus dorsi muscle could promote calcification and vascularization in tissue-engineered bone, when used separately or in combination.
出处 《中华口腔医学杂志》 CAS CSCD 北大核心 2007年第7期436-437,共2页 Chinese Journal of Stomatology
基金 国家自然科学基金(30471899)
关键词 组织工程 富血小板血浆 Bones Tissue engineering Platelet rich plasma
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