survivin基因在膀胱癌中的表达及其临床意义

Expression of survivin mRNA and its clinical implications in bladder cancer

目的:探讨survivin基因在膀胱癌组织中的表达情况及其临床意义。方法:采用RT-PCR方法检测膀胱癌和正常膀胱黏膜中survivin基因的表达情况,分析其表达与膀胱癌的分期、分级及复发的关系。结果:sur-vivin mRNA在膀胱癌中总阳性表达率为78.6%(33/42)。在浸润性膀胱癌中阳性表达率(95.7%)较浅表性膀胱癌中阳性表达率(57.9%)明显升高;在复发性膀胱癌中,survivin mRNA阳性表达率(95.0%)较初发膀胱癌的阳性表达率(63.6%)显著升高(P<0.05);survivin mRNA在正常膀胱黏膜中均无表达。结论:作为敏感性较强的生物瘤标,survivin基因 mRNA表达可用来预测膀胱癌的复发和浸润。

Objective：To investigate the expression of survivin mRNA in carcinoma of bladder and it＇s clinical implications. Methods：Expression of survivin mRNA was detected by RT-PCR, and it＇s relations with the clinicopathologic parameters in bladder cancer was analyzed. Results： The total rate of survivin mRNA expression in patients with bladder carcinoma was 78. 6% （33/42）. The expression rate of survivin mRNA in infiltrated tumor （95 % ）（22/23） was significantly higher than that in superficial bladder cancer （57.9 % ）（ 1 1/19）, and the expression rate of survivin mRNA in relapsed tumors （95.0%）（19/20） was much higher than that in initial bladder cancers （63.6%）（14/22）（P〈0.05）. Survivin mRNA was not detected in normal mucosa of bladder in the control group. Conclusions：As a sensitive biomarker for bladder cancer, the expression of survivin mRNA can serve as an indicator to predict recurrence and infiltration in bladder cancer.