不同环境对局灶性脑梗死大鼠梗死灶周围皮质GFAP、GAP-43表达的影响

Effects of different environmental intervention on GFAP and GAP-43 expression in rats after unilateral local cerebral infarction

目的:观察不同环境对局灶性脑梗死大鼠胶质纤维酸性蛋白(GFAP)和生长相关蛋白-43(GAP-43)表达的影响。方法:SD大鼠95只,采用电凝法造成右侧大脑中动脉阻断(MCAO)模型后,随机分为独居组(n=20)、社交组(n=30)、探索学习组(n=20)、丰富环境组(n=20)、假手术对照组(n=5)。于不同时点分批处死大鼠,用免疫组化染色观察梗死灶周围皮质GFAP和GAP-43阳性细胞数。结果:梗死灶周围皮质GFAP和GAP-43阳性细胞数第1d时即增多,1—4周时探索学习组和丰富环境组阳性细胞数目明显多于独居组和社交组;社交组1—4周时GFAP阳性细胞数多于独居组,1—2周时GAP-43阳性细胞数多于独居组;第4周时GFAP阳性细胞数,丰富环境组高于探索学习组。结论:丰富环境、探索学习及社会交往均能促进梗死灶周围皮质GFAP和GAP-43表达。

Objective： To observe effects of different environmental intervention on GFAP and GAP-43 expression in rats after unilateral local cerebral infarction. Method： After making the model of middle cerebral artery occlusion （MCAO） by electric coagulation successfully, 95 male rats were randomly divided into individual living group（n=20）, social communication group（n=30）, learning group（n=20） , enriched environment group（n=20） and sham operated group（n=5）. The rats were randomly sacrificed at different time points. The expression of GFAP and GAP- 43 in peri-ischemic cortex were examined by immunohistochemistry staining. Result： The number of GFAP labeled cells and GAP-43 labeled cells in peri-ischemia cortex in enriched environment group and learning group were higher than that in other two groups （P〈0.01 or P〈0.05）, it was also higher in social communication group than that in individual living group （P〈0.01 or P〈0.05）. Conclusion： Enriched environment, learning and social communication could enhance GFAP and GAP-43 expression in rats after unilateral local cerebral infarction.