小鼠肝癌微波消融对宿主免疫细胞和细胞因子的影响

Effect of microwave ablation on immunocyte and cytokine production in hepatocellular carcinoma mice

目的探讨小鼠肝癌微波消融(microwave ablation,MA)对宿主免疫细胞组成和细胞因子分泌的影响。方法建立C57BL/6J小鼠Hepa1-6细胞移植肝癌模型,对肿瘤进行微波消融或手术切除,分别用流式细胞仪和ELISA法检测治疗前后宿主周围血中的CD4+、CD8+、NK1.1+细胞组成(%)及血浆细胞因子IL-2、IL-10、IL-12、IFN-γ水平(ng/L)。结果荷瘤2～3周组鼠与正常组鼠比较,免疫细胞和细胞因子区别不显著。MA组较荷瘤组CD8+、CD4+T细胞及IL-2、IL-10无明显差别,NK1.1+细胞明显升高(P<0.05,n=6),IL-12、IFN-γ显著上升(均P<0.01,n=6)。手术组比荷瘤组免疫细胞和细胞因子也无明显变化。MA组比手术组CD8+、CD4+T细胞及IL-2、IFN-γ、IL-10无明显区别,NK1.1+细胞、IL-12明显上升(分别P<0.01、P<0.05,n=6)。结论MA原位灭活小鼠肝癌可促进Th2/Th1偏移及NK细胞组成的显著升高。

Objective To investigate the effect of microwave ablation （MA） of hepatocellular carcinoma （HCC）, which influences the proportion of immunocytes and cytokine-secreting of the tumor- bearing mice. Methods A HCC model of C57BL/6J mouse was established by subtaneous inoculation of syngeneic Hepal-6 cells, the tumors were treated with MA or resection before and after the treatment, the percentage of CD4^＋ , CD8^＋ , NK1.1^＋ cell of peripheral blood, and the concentration of IL-2, IL-10, IL-12 and IFN-γof plasma of the hosts were determined with flow eytometry and ELISA, respectively. Results In group 2 - 3 weeks tumor- bearing, the proportion of the immunoeytes detected, and the concentration of the examined cytokoines was not markedly altered. For comparison of group MA with that tumor-bearer,CD4^＋ ,CD8^＋ cells and IL-2,IL-10 of the former were not obviously different from those of the latter, NK1.1^＋ cell, IL-12 and IFN-γ of the former markedly higher than that of the latter （P 〈0. 05, P 〈0. 01, P 〈0. 01 ,respectively,n =6）. Compared with the group tumor-bearing, the immuocytes and cytokines of the group resection were not significantly changed. The NK1.1^＋ cells and IL-12 of group MA were markedly higher than that of group resection（P 〈0. 01 and P 〈0. 05, respectively, n = 6）, but there was no significant difference for other immune cells and cytikines in the two groups. Conclusion The MA of the mouse HCC may induce Th2/ Th1 deviation and markedly enhance the proportion of NK ceils of the tumor-bearer.