摘要
目的:研究白蛋白纳米球的制备工艺,并检测偶联单克隆抗体白蛋白纳米球对靶细胞的特异性杀伤作用。方法:采用高温固化法制备白蛋白纳米球,通过正交设计筛选最佳工艺。制备免疫白蛋白纳米球,观察白蛋白纳米球对急性早幼粒细胞白血病M3亚型白血病细胞的特异性杀伤作用。结果:在最佳工艺条件下制备白蛋白纳米球,平均直径为250nm左右,形态圆整。免疫纳米球(0.3μmol/mL剂量组作用48h)对靶细胞(急性早幼粒细胞白血病M3亚型白血病淋巴细胞)杀伤率为(34.12±4.76)%,对正常对照(正常人外周血淋巴细胞)杀伤率仅为(5.37±2.21)%。结论:免疫纳米球对靶细胞具有特异性杀伤作用,而对非靶细胞的杀伤作用较小。
Objective : to study the preparation technology of HAS - nanoparticles, and investigate the peculiar toxicity of HAS - nanoparticles on targeting cells. Methods : we studied the best preparing formula of HAS - nanoparticles by the orthogonal design test. Subsequently HAS - nanoparticles containing arsenous oxide prepared by emulsion - heat stabilization technique were conjugated monoclonal antibodies. Finally the antitumor efficacy of HAS - nanoparticles beating Monoclonal antibody was investigated in M3 acute promyelocytic leukaemia lymphocytes. Results : We got the best formula ,which show the entrapping rate and loading of arsenous oxide was higher and the average particle size was about 250nm. In the study we observed that the death rate of targeting cells after action for 48h hours was 34.12 percent when the dose of drug is 0.3μmol/mL, whereas that of control - group cells was only 5.37 percent. Conclusion : In conclusion, drug targeting can be achieved by incorporating monoclonal antibody on the surface of HAS - nanoparticles.
出处
《中华中医药学刊》
CAS
2007年第8期1633-1635,共3页
Chinese Archives of Traditional Chinese Medicine
基金
江西省科技厅重大专项课题(20041A0300203)
关键词
AS2O3
白蛋白纳米球
靶向抗癌药
arsenous oxide
HAS - nanoparticles
monoclonal antibody
targeting antitumor drugs