组蛋白甲基转移酶基因G9a在肝外胆管癌组织中转录表达及其临床意义的研究

Transcriptional expression of histone methyltransferase gene G9a and its clinical significance in extrahepatic cholangiocarcinoma

目的探讨组蛋白甲基转移酶基因G9a在肝外胆管癌中的表达及其临床意义。方法用RT-PCR的方法检测48例肝外胆管癌组织及39例癌旁对照组织中C9a mRNA的表达，并分析其阳性表达率与临床病理因素之间的相关性。结果G9a在肝外胆管癌组织中的转录表达率为56．25％（27／48），明显高于对照组织并与肝外胆管癌的淋巴转移及TNM分期（P〈0．05）相关。结论组蛋白甲基转移酶基因G9a在胆管癌组织中表达上调，提示由G9a引起的组蛋白甲基化紊乱在肝外胆管癌的发生发展中发挥重要作用。

Objective To investigate the transcriptional expression of histone methyltransferase gene Gga and its clinical significance in extrahepatic cholangiocarcinoma. Methods The mRNA of Gga was detected by RT-PCR in 48 extrahepatic cholangiocarcinomas and 39 contral tissues. Results The frequency of transcriptional expression of Gga in extrahepatic cholangiocarcinoma was 56. 25% （27/ 48） ,and it was higher than that in the control and positively correlated with lymph metastasis and TNM stage （P〈0.05）. Conclusion The significant difference in expression of G9a between tumor and control implicated the important roles of histone methylation disruption resulted from increased Gga expression in extrahepatic cholangiocarcinoma.