电针内关穴对AMI大鼠自主神经与中枢P物质、一氧化氮合酶的影响

Effect of electroacupuncture of Neiguan point on autonomic nerve,SP and NOS in AMI rats

目的:探讨电针大鼠内关穴抗急性心肌缺血(AMI)损伤的自主神经效应路径和可能的有效物质。方法:SD大鼠随机分为正常组(A组)、模型对照组(B组)、电针内关组(C组),每组6只。各组分离颈部右侧迷走神经、左侧交感神经,BL-420E+生理仪记录放电情况。B、C组Pit股静脉注射制作AMI模型,C组造模后5min电针左侧内关穴治疗。最后免疫组化ABC法、NADPH-黄递酶组化法分别检测各组大鼠下丘脑室旁核(PVN)、延髓迷走神经复合区及脊髓(C8-T2)外侧角的P物质(SP)和一氧化氮合酶(NOS)的表达。观测各组大鼠心交感神经、迷走神经放电频率变化以及治疗前后自主神经SP、NOS灰度值。结果:造模前A、B、C组迷走神经放电频率分别为(5.12±0.67)、(5.13±0.36)、(5.05±0.47)次/min,交感神经放电频率分别为(23.21±3.23)、(22.84±2.18)、(23.04±4.32)次/min,组间比较均无差异;造模后0min时B、C组迷走神经放电频率增加、交感神经放电频率减少,与A组比较有显著差异(P<0.05);造模后20minC组迷走神经和交感神经放电频率恢复明显,与A组比较均无差异,与B组比较均有差异(P<0.05)。A组PVN、延髓迷走神经复合区、脊髓(C8-T2)外侧角的SP灰度值分别为(115.44±12.20)、(84.64±14.50)、(43.32±12.14),NOS灰度值分别为(98.03±14.72)、(64.60±12.33)、(40.33±10.42),B组各部位SP灰度值小于A组,组间有差异性(P<0.05),C组各部位SP灰度值均高于A、B组,组间比较有差异性(P<0.05);B组各部位NOS灰度值增加(P<0.05),C与A组各部位NOS灰度值无明显差异。结论:电针内关穴对AMI大鼠的保护效应与自主神经及中枢SP、NOS的协调作用密切相关。

Objective： To study autonomic nerve pathway and possible effective substance of injury resistance of electroacupuncture of neiguan point in AMI rats. Methods： SD rats were divided into three groups： normal group （group A）, control group （group B） and electroacupuncture of Neiguan point group （group C） at random. Each group had 6 rats and the right cranial nerve and left sympathetic nerve were isolated. BL-420E ＋ physiograph was used to record electrical discharge. AMI models were established by Pit vena femoralis injection in group B and group C. After 5 minutes of the model establishment, rats of group C were treated by electroacupuncture of neiguan point. Finally, immunohistochemical ABC method and there are histological chemistry method, NADPH-diaphorase histo- chemical method were used to detect the express of paraventricular hypothalamic nucleus （PVN）, complexity of bulb and cranial nerve, spinal lateral crus of substance P（SP） and nitricoxide synthase （NOS）. The discharge frequency of adrenergic nerve and erarlial nerve, and OD of SP and NOS of autonomic nerve of each group were observed. Results： Before the models were established, the discharge frequency （Hz） of cranial nerve were （5.12 ±0.67）, （5.13 ±0.36）, （ 5.15 ± 0.47） times/min and t hat of sympathetic nerve were （ 23.21 ± 3.23 ）, （ 22.84 ± 2.18 ）, （ 23.04 ±4.32）times/min in each group. There was no significant difference in the three groups. At 0 minute after modelswere established, compared with group A, the discharge frequency of cranial nerve increased obviously and the discharge frequency of sympathetic nerve decreased significantly of both of group B and group C （P 〈 0.05）. At 0 minute after models were established, the discharge frequency of cranial nerve and sympathetic nerve of group C recovered obviously. There was on difference with group A and significant difference with group B （ P 〈 0.05）. In group A, the OD of SP in paraventricular hypothalamic necleus（PVN）, bulb and cranial nerve complex, spinal lat- eral crus were（ 115.44±12.20）, （84.64± 14.50）, （43.32 ± 12.14）and the OD of NOS in paraventricular hypothalamic necleus（ PVN ）, bulb and cranial nerve complex, spinal lateral crus were（98.03 ±14.72）, （ 64.60± 12.33）, （40.33 ±10.42）. Compared with group A, the OD of SP in all parts of group B decreased obviously （ P 〈 0.05）. Compared with group A and group B, the OD of SP of group C increased （P 〈 0.05）. Compared with group A and group C, the OD of NOS of group B increased （ P 〈 0.05）. There was no difference between group C and group A. Conclusion： The protecting mechanism of electroacupuncture of neiguan point in AMI rats has close relationship with coordination of SP and NOS in autonomic and central nerve.