摘要
目的探讨支气管哮喘时神经生长因子(NGF)对Th1/Th2类细胞因子分泌的调控作用。方法随机将SD大鼠分为4组:正常对照组、哮喘组(建立哮喘模型)、外源性NGF干预组(NGF组)、抗NGF抗体干预组(抗NGF抗体组),每组8只。14d后取肺组织进行HE染色,观察病理改变;免疫组化和酶联免疫吸附试验(ELISA)检测肺组织的NGF蛋白,Th1类细胞因子γ-干扰素(IFN-γ)、Th2类细胞因子白细胞介素4(IL-4)蛋白水平。结果哮喘组支气管、肺组织炎症明显;肺组织NGF蛋白的分泌水平和免疫阳性信号也显著增高(P<0·001);IL-4蛋白水平明显增强(P<0·05),而IFN-γ蛋白水平明显减低(P<0·001);肺组织中的NGF蛋白与IFN-γ/IL-4蛋白比值呈显著负相关(P<0·01)。NGF组更进一步增强上述变化(P<0·05),而抗NGF抗体干预组显著缓解上述变化(P<0·05)。结论NGF可诱导哮喘大鼠神经源性炎症,并分别通过增强Th2类细胞因子分泌,减弱Th1细胞因子分泌,诱导哮喘Th1/Th2免疫失衡效应。
Objective To study the effect of nerve growth factor（NGF） on secretion of Th1/Th2 cytokines. Methods （1）32 SD rats were divided into 4 groups randomly： asthma （the asthmatic models were established）, control, NGF, anti-NGF-antibody groups. After 14 days the lung tissues were examined for pathologic changes by HE staining. NGF in the lungs was determined by enzyme-linked immunosorbent assay （ELISA） and immunohisto- chemistry assay. The level of interferon-γ（IFN-γ, one of the Th1 cytokines） and interleukin-4 （IL-4, one of the Th2 cytokines） was detected by ELISA. Results In the asthma group, more inflammatory cells were detected in the lungs, more NGF-immunoreactive infiltrating ceils and higher levels of NGF （ P 〈 0. 001 ） in the lungs were recorded. The level of IL-4 （P 〈 0. 05 ） was enhanced and the levels of IFN-γ（ P 〈 0. 001 ） were decreased significantly in the lungs. In the lungs of the asthma group, a significant negative correlation was found between NGF and the ratio of IFN-γand IL-4（P 〈 0. 01 ）. In the NGF group, the changes were significant than those in the asthma group （P 〈 0. 05 ）. In the the anti-NGF-antibody group, the changes were decreased than those in the asthma group （P 〈 0. 05 ）. Conclusion In addition to inducing neurogenic inflammation in asthmatic rats, NGF may amplify Th1/Th2 imbalance in asthma by enhancing Th2 cytokines and decreasing Th1 cytokines.
出处
《基础医学与临床》
CSCD
北大核心
2007年第8期872-875,共4页
Basic and Clinical Medicine
基金
湖南省卫生厅基金(B2003-035)
