濒危植物夏蜡梅黄酮化合物薄层色谱分析

Analysis of Flavonoids in Endangered Plant Sinocalycanthus chinensis Using Thin Layer Chromatography

采用薄层层析法对不同居群夏蜡梅(Sinocalycanthus chinensis)叶、同一居群夏蜡梅不同营养器官中的黄酮化合物进行分离,并以芦丁为标准品通过双波长锯齿扫描作定量分析。结果表明:不同居群夏蜡梅叶片中黄酮化合物含量以鸡窝坪居群最高,中坪居群次之,龙塘山居群最低,除双石边和白水坞两个居群差异不显著外,其它各居群间差异均达到显著水平;同一居群夏蜡梅黄酮化合物在不同营养器官中含量不同,叶片中含量显著高于其它营养器官,根、茎、一年生枝和二年生枝等4个营养器官中黄酮化合物含量差异不显著。薄层层析法分离黄酮化合物组分结果显示,除大明山居群叶片黄酮化合物为5种组分外,其它居群叶片均为7种组分;大明山居群夏蜡梅的根、茎、一年生枝和二年生枝等营养器官黄酮化合物均为6种组分,叶片中缺少Rf值为0.66的化合物。

The flavonoids in the leaves of Sinocalycanthus chinensis from different populations and those in the different organs from the same population were extracted and separated by using polyamide TLC-chromatography. Every component was quantitatively analyzed by dual wavelength flying scanning using rutin as the standard sample. The results showed as the following. The content of flavonoids in Jiwoping population was the highest,while that in Zhongping population the second and that in Longtangshang population the lowest. There was significant difference among pair-wise populations,except for that between Shuangshibian population and Baishuiwu population. In the same population, the content of flavonoids in different organs was different. The content of flavonoids in the leaves was significantly higher than those in other organs. There was no significant difference among stems, one year twigs and two years twigs. Based on the analysis of TLC chromatography,seven components were found in the flavonoids extracted from the leaves except that there were only 5 components found in the flavonoids extracted from the leaves in Damingshan population. Six components were found in the flavonoids extracted from the roots, stems, one year twigs and two years twigs. The component with Rf equal to 0. 66 was absent in leaves.