毛细支气管炎患者血清IL-13表达及其与病情相关性探讨

Serum interleukin-13 levels in infants with acute bronchiolitis

目的探讨IL-13水平在急性期毛细支气管炎(简称毛支)中的表达及其与病情的相关性。方法急性期毛支患儿42例(其中轻症组22例,重症组20例),正常对照组16例。采用双抗体夹心酶联免疫吸附试验(ELISA法),检测血清中IL-13的水平。结果急性毛支患儿血清IL-13的含量为(6.68±2.12)ng/L。其中轻症组(7.11±1.95)ng/L,重症组(6.64±2.24)ng/L。两组比较差异无统计学意义(P>0.05),而急性期毛支患儿与正常对照组比较,差异有统计学意义(P<0.05)。结论急性期毛支患儿血清水平均增高。与对照组比较差异有统计学意义。但轻、重各组比较,差异无统计学意义,提示IL-13参与了毛支的发病过程,但lL-13不能反映病情的严重程度。

Objective To observe the change of serum interleukin-13（IL-13） and the clinical severity of disease in infants with acute bronchiolitis. Methods Forty-two infants with acute bronchiolitis from 2 to 12 months of age were put into this study and divided into two groups according to the clinical severity score., the mild bronchiolitis group and the severe bronchiolitis group： 16 healthy infants were selected as the control group. IL-13 level in serum was detected with Sandwich enzyme linked immurosorbent assay （ELISA）. Results The serum level of IL-13 in acute bronchiolitis patients （6.68±2.12）ng/L was significantly higher than that in the control group （5.48 ± 1.28）ng/L（P〈 0.05）. The mean IL-13 level showed no statistical difference between the mild group and severe group [（7.11± 1.95） ng/L vs （6.64±2.24）ng/L,P〉0.05]. Conclusion The serum level of IL-13 increases in acute bronchiolitis and may involve in the pathogenesis of the disease. But, there is no relationship between the serum concentrations of IL-13 and clinical severity of the disease.