辣椒子叶再生体系的建立

Establishment of Regeneration System of Pepper Cotyledon

以保加利亚尖椒和赤研6号椒子叶为试材,探讨植物生长调节剂及其组合(LC与IAA)对不定芽诱导分化及伸长的影响。结果表明,LC 1.0mg/L+IAA 0.1mg/L和LC 1.0mg/L+IAA 1.0mg/L均有利于保加利亚尖椒不定芽的分化,分化率均达100%,优胜芽率分别为83.3%和66.7%;LC 0.1mg/L+IAA 0.2mg/L有利于保加利亚尖椒不定芽的伸长,伸长率达78.5%,而LC 0.2mg/L+IAA 0.6mg/L有利于赤研6号椒不定芽的伸长,伸长率达58.5%;1/2 MS+IAA 1.0mg/L+NAA 0.5mg/L有利于不定芽的生根,保加利亚尖椒和赤研6号椒生根率分别为100%和85%。

Effects of plant regulators on adventitious buds differentiation and elongation of Bulgaria pointed pepper and chiyan No.6 were studied in the report. The results showed that the best combinations for the adventitious buds differentiation of Bulgaria pointed pepper and chiyan No.6 were LC 1.0mg/L ＋ IAA 0.1mg/L and LC 1.0mg/L ＋ IAA 1.0mg/L, respectively, with which the differentiation rates were all 100 %, the rates of superior buds were 83.3 % and 66.7 %, respectively. Best combinations for elongation of adventitious buds of Bulgaria pointed pepper and chiyan No.6 were LC 0.1mg/L ＋ IAA 0.2mg/L and LC 0.2mg/L ＋ IAA 0.6mg/L, respectively, the elongation rates were 78.5% and 58.5%, respectively. The optimum rooting medium was 1/2 MS ＋ IAA 1.0mg/L ＋ NAA 0.5mg/L, and the average rooting rates were 100% and 85%, respectively.