一株H3N2亚型猪流感病毒广东分离株的基因组序列分析

Genome sequence analysis of an H3N2 subtype swine influenza virus isolated from Guangdong province in China

从广东省疑似流感发病猪分离到1株H3N2亚型猪流感病毒（A/Swine/Guangdong/01/2005（H3N2））,对其各个基因进行克隆与测序,并与GenBank中收录的其它猪流感、禽流感和人流感的相关基因进行比较,结果表明,HA全基因与广东2003~2004年分离的H3N2猪流感毒株的核苷酸序列同源性在99%以上,与纽约90年代末分离的H3N2人流感毒株同源性在98.5%以上;NA基因与纽约1998~2000年分离的H3N2人流感毒株的核苷酸序列同源性在99%以上;NS基因、M基因的核苷酸序列与H1N1亚型猪流感毒株A/swine/HongKong/273/1994（H1N1）的核苷酸序列同源性较高,分别为97.9%、98.4%,与美洲A/swine/Iowa/17672/1988（H1N1）的核苷酸序列同源性分别为96.7%、97.1%;其他基因的核苷酸序列与H3N2人流感毒株具有很高的同源性。因此,推测其M和NS基因来源于H1N1亚型猪流感病毒,HA、NA及其他基因均来源于H3N2亚型人流感病毒。表明此H3N2亚型猪流感病毒为H3N2亚型人流感病毒和H1N1亚型猪流感病毒经基因重排而得到的重组病毒。

An H3N2 subtype swine influenza virus,A/Swine/ Guangdong/01/2005（H3N2）,was isolated from pigs with influenza-like signs in Guangdong province in 2005. Reverse transcription-polymerase chain reaction （RT-PCR） was used to amplify the gene segments for sequencing analysis. Phylogenetic analysis showed that the hemagglutinin（HA） gene of A/Swine/ Guangdong/01/2005 shared high degree of sequence identity with those of H3N2 viruses isolated from swine in Guangdong province from 2003 to 2004 and H3N2 viruses isolated from human in New York in the end of 1990s. The neuraminidase （NA） gene of this H3N2 swine virus had high degree of sequence identity with those of H3N2 viruses isolated from human in New York from 1998 to 2000. While the nonstructure （NS） gene and matrix （M） gene of this H3N2 swine virus showed high degree of sequence identity with those of classical H1N1 swine influenza viruses. These results indicated that the M and NS gene might originate from the H1N1 subtype swine influenza viruses,while the HA,NA and other genes of this H3N2 subtype swine influenza virus might originate from the H3N2 subtype human influenza viruses. Therefore,the H3N2 swine influenza virus,A/Swine/Guangdong/ 01/2005（H3N2）,is a recombinant of H3N2 human influenza virus and classical H1N1swine influenza virus.