摘要
目的探讨丹参酮ⅡA纳米微粒(tanshinoneⅡA nanoparticle,TS-NP)治疗小鼠肝癌的作用及机制。方法采用乳化溶剂挥发法(O/W)制备TS-NP。建立小鼠肝癌模型,随机分为生理盐水组、空白纳米组(blank nanoparticle,B-NP)、丹参酮ⅡA组(tanshinoneⅡA,TSⅡA)和TS-NP低、中、高3个剂量组(TS-NP L、TS-NP M、TS-NP H),分别尾静脉给药。治疗第8天处死小鼠,比较各组肿瘤重量、肿瘤坏死程度。采用TUNEL标记法检测细胞凋亡率,免疫组化S-P法检测周期蛋白E(Cyclin E)的表达。结果治疗后与生理盐水组比较,TSⅡA组、TS-NP各剂量组瘤体重量显著降低(P<0.01)TS-NP组瘤体重量明显低于等剂量的TSⅡA组(P<0.01),肿瘤坏死明显(P<0.01),肝癌细胞凋亡率升高(P<0.01),Cyclin E的表达降低(P<0.01)。结论TS-NP能够抑制小鼠肝癌生长,丹参酮ⅡA纳米的疗效优于等剂量的丹参酮ⅡA;其治疗肝癌的机制可能与抑制Cyclin E表达从而抑制肿瘤细胞增殖、诱导细胞调亡有关。
Objective To investigate the mechanism of Tanshinone Ⅱ A nanoparticles (TS-NP)-induced apoptosis on hepatoma in mice. Methods TS-NP was prepared with O/W, hepatoma model in mice was established. Mice with hepatoma were randomly divided into six groups: control group (NS), blank nanoparticles (B-NP) group, Tanshinone Ⅱ A nanoparticles low dose (TS-NP L) group, middle dose(TS-NP M) group, high dose (TS-NP H) group. Separately NS, B-NP, TS Ⅱ A, TS-NP L, TS-NP M, TS-NP H were infused via tail vein. On the 8th day after treatment, and the mice were sacrificed. The weight and necrosis grade of tumor in each group were compared. The cell apoptosis index was tested by TUNEL; and the expression of Cyclin E was detected by immunohistochemical technique (S-P method). Results The weight of TS Ⅱ A and TS-NP group was significantly lower than that of NS group (P〈0.01) and tumor necrosis was more serious. The apopoptic index in TS Ⅱ A and TS-NP group was higher than those of other groups (P〈0.01), but the expression of Cyclin E was lower (P〈0.01). Conclusion TS-NP can inhibit the growth of tumor in mice; and the curative effect of Tanshinone Ⅱ A nanoparticles was better than that of Tanshinone Ⅱ A with the same dose. The mechanism of TS-NP on hepatoma may be associated with decreased expression of Cyclin E to inhibit the cell proliferation and promote the apoptosis.
出处
《上海中医药杂志》
北大核心
2007年第9期74-77,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市中医药科研基金资助项目(2004J012A)
上海市医学重点专科建设项目(05Ⅱ016)