含C-src同源序列的酪氨酸磷酸酶SHP-2和SHP-1在前列腺增生组织重构中的表达

Expression of tyrosine phosphatase containing C-src homology SH-2 in benign prostate hyperplasia

目的:探讨含C-src同源序列的酪氨酸磷酸酶SHP-2和SHP-1在前列腺增生组织重构中的表达及意义。方法:采用免疫组化En Vision二步法检测10例正常前列腺组织、30例良性前列腺增生组织(BPH)、20例前列腺上皮内瘤变(PIN)、20例高分化前列腺癌(Pca)和10例低分化前列腺癌组织中SHP-2和SHP-1的表达。结果:SHP-2在正常组中主要表达在基底和上皮细胞的胞浆中,少数在胞核中表达;在BPH组中基底和上皮细胞的胞浆和胞核中均有表达;在PIN、高分化Pca和低分化Pca组中主要在细胞核内表达,少数在胞浆中表达。SHP-2在各组的平均染色指数分别为0.4、1.7、2.1、2.2和2.6。SHP-1在正常前列腺组织,BPH、PIN和高分化Pca的组织细胞的胞浆中均有不同程度表达,在低分化Pca组织中不表达;SHP-1在各组的平均染色指数分别为1.8、1.8、1.5、1.2和0.4。结论:在前列腺组织细胞增殖、分化及转化过程中,存在SHP-1和SHP-2相关的信号传导的改变。SHP-2异常激活和表达部位的改变可能是导致前列腺组织重构、增生及癌变的重要机制。

Objective： To explore the expression of tyrosine phosphatase containing C-src homology SH-2 （SHP-1 and SHP-2） in benign prostate hyperplasia. Methods： With En Vision two-step method,the expression of SHP-1 and SHP-2 was detetecd in 10 cases of normal prostate tissue, 30 cases of BPH, 20 cases of PIN, 20 cases of high differential Pca and 20 cases of low differential Pca. Results： The expression of SHP-2 in normal group was mainly distributed in the cytoplasm of secretive cells and basal cells, and a little part in the nucleu. In BPH it was distributed equally in the plasm and nucleu. In PIN ,high differential Pca and low differential Pca, SHP-2 expressed mainly in nucleu. The average dyeing index of SHP-2 in each group is 0.4,1.7,2. 1,2. 2 and 2. 6. SHP-1 positive expression in normal prostate,BPH, PIN and high differential Pea showed differentiating layer staining in the cytoplasm of secretive cells and basal cells,while not in low differential Pea. The average dyeing index of SHP-1 in each group is 1.8,1.8,1.5,1.2 and 0.4. Conclusions. There are transformation in signal transduction relation with SHP-1 and SHP-2 in the progress of prostate cell proliferation,differentiation and malignant. The abnormal activation and distribution of SHP-2 might induce prostate reconstruction and hyperplasia,even carcinoma.