独活寄生汤对荷瘤小鼠的抗肿瘤作用研究

An Experimental Study on the Anti-tumor Effects of Duhuojisheng Decoction in Tumor Bearing Mice

目的：探讨独活寄生汤（DHJSHD）对小鼠肉瘤S180的抑制作用及其机制。方法：以S180荷瘤小鼠为模型,观察独活寄生汤对荷瘤鼠瘤重的影响和肿瘤组织病理学改变,用改良的MTT法测定荷瘤鼠主要免疫指标。结果：独活寄生汤能显著抑制S180瘤重,抑瘤率为32.45%～43.75%;肿瘤组织有明显的出血坏死及炎细胞浸润,同时独活寄生汤活化荷瘤鼠的T细胞增殖能力,促进其自然杀伤细胞（NK）活性和白介素-2（IL-2）分泌水平。结论：独活寄生汤能抑制小鼠肉瘤S180的生长,其机制可能与调节机体免疫功能有关。

Objective：To study the anti-tumor effects of Duhuojisheng Decoction on mice bearing sarcoma 180 and its possible mechanism. Methods： S180 bearing mice as model ,effects of Duhuojisheng Decoction on tumor weight and the pathological diagnosis of tumor tissue were investigated. Modified MrIT assay was used to determine immunological function in bearing mice. Results： Duhuojisheng Decoction could markedly inhibit the tumor weight of S180 ,the rate of inhibition ranged from 32.45 -43.75 percent. Duhuojisheng Decoction could prompt tumor tissue large area necrosis and inflammation cell infiltration, activate proliferating ability of T cells, markedly increase activity of natural killers（NK） and secretion of interleukin-2 （IL-2）. Conclusion： Duhuojisheng Decoction had anti-tumor effects by regulating immune system.