p16蛋白在胰腺癌组织中的表达及意义

Expression and Significance of P16 protein in Pancreatic Adenocarcinoma

目的通过检测p16蛋白在胰腺癌组织中的表达,探讨其临床意义。方法应用链霉菌抗生物素-过氧化酶(Streptavidin-Peroxidase,SP)免疫组化法对32例原发性胰腺导管腺癌、12例正常胰腺组织进行p16蛋白检测。结果低分化组胰腺癌组织中p16蛋白的缺失率高,与高分化组和正常胰腺组织组之间差异有显著性(P<0.05);中分化组胰腺癌组织中p16蛋白的缺失率与正常胰腺组织组之间差异有显著性(P<0.05)。转移组胰腺癌组织中p16蛋白的缺失率与无转移组之间差异也有显著性(P<0.05)。结论p16蛋白的表达能反映胰腺癌的生物学行为。

Objective To study the biological significance through the detection of the expression of P16 protein in pancreatic adenocarcinomas（PC）. Methods P16 proteins were detected by SP immunohistochemical method in formalin - fixed paraffin - embedded samples from 32 pancreatic adenocarcinomas（ PC ）, 12 normal pancreatic tissues which were confirmed pathologically as normal pancreatic tissue. Results Loss of p16 expression was higher in poorly - differentiated PC, there were significant differences （P 〈 0.05 ）between poorly -differentiated PC and well -differentiated PC and normal pancreatic tissue ; and there were significant differences between middle - differentiated and normal pancreatic tissue （P 〈 0. 05 ） . Significant differences were found between the group with lymph node metatasis and the group without lymph node metatasis. Conclusion The expression of P16 might reflect the biological significance of PC.